A cellular logic circuit for the detection of bacterial pore-forming toxins

被引：8

作者：

Zhu, Kui ^{[1
]}

Acaroez, Ulas ^{[1
]}

Maertlbauer, Erwin ^{[1
]}

机构：

[1] Univ Munich, Fac Vet Med, Inst Food Safety, D-85764 Oberschleissheim, Germany

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 45期

关键词：

BACILLUS-CEREUS; BIOSENSORS; CELLS; REVEALS; MODEL;

D O I：

10.1039/c3cc41932k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present a cellular logic circuit for deciphering the profiles of toxin production in B. cereus, using multiple readout techniques based on the pore formation on the cell membrane. This new assay enables the simultaneous detection of seven biomarkers in pathogenic strains from various samples.

引用

页码：5198 / 5200

页数：3

共 31 条

[1] From soil to gut:: Bacillus cereus and its food poisoning toxins [J].

Arnesen, Lotte P. Stenfors ;

Fagerlund, Annette ;

Granum, Per Einar .

FEMS MICROBIOLOGY REVIEWS, 2008, 32 (04) :579-606

[2] Pore-forming toxins and cellular non-immune defenses (CNIDs) [J].

Aroian, Raffi ;

van der Goot, F. G. .

CURRENT OPINION IN MICROBIOLOGY, 2007, 10 (01) :57-61

[3] Molecular AND logic gate based on bacterial anaerobic respiration [J].

Arugula, Mary Anitha ;

Shroff, Namita ;

Katz, Evgeny ;

He, Zhen .

CHEMICAL COMMUNICATIONS, 2012, 48 (82) :10174-10176

[4] Programmable single-cell mammalian biocomputers [J].

Auslaender, Simon ;

Auslaender, David ;

Mueller, Marius ;

Wieland, Markus ;

Fussenegger, Martin .

NATURE, 2012, 487 (7405) :123-+

[5]

Beecher DJ, 1997, J BIOL CHEM, V272, P233

[6] Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study [J].

Bielaszewska, Martina ;

Mellmann, Alexander ;

Zhang, Wenlan ;

Koeck, Robin ;

Fruth, Angelika ;

Bauwens, Andreas ;

Peters, Georg ;

Karch, Helge .

LANCET INFECTIOUS DISEASES, 2011, 11 (09) :671-676

[7] Pathogenic Pore-Forming Proteins: Function and Host Response [J].

Bischofberger, Mirko ;

Iacovache, Ioan ;

van der Goot, F. Gisou .

CELL HOST & MICROBE, 2012, 12 (03) :266-275

[8] Membrane injury by pore-forming proteins [J].

Bischofberger, Mirko ;

Gonzalez, Manuel R. ;

van der Goot, F. Gisou .

CURRENT OPINION IN CELL BIOLOGY, 2009, 21 (04) :589-595

[9] Tracking, tuning, and terminating microbial physiology using synthetic riboregulators [J].

Callura, Jarred M. ;

Dwyer, Daniel J. ;

Isaacs, Farren J. ;

Cantor, Charles R. ;

Collins, James J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (36) :15898-15903

[10] DNA computing: applications and challenges [J].

Ezziane, Z .

NANOTECHNOLOGY, 2006, 17 (02) :R27-R39

← 1 2 3 4 →