A portable fluorescence resonance energy transfer biosensor for rapid detection of silver ions

被引：17
作者：
Chen, Yi-Ju [1 ]
Liu, Cheng-You [1 ]
Tsai, Dong-Yu [1 ]
Yeh, Yi-Chun [1 ]
机构：
[1] Natl Taiwan Normal Univ, Dept Chem, 88,Sect 4,Tingzhou Rd, Taipei 11677, Taiwan
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2018年 / 259卷
关键词：
Fluorescence resonance energy transfer; FRET; CupR; Silver biosensor; METAL-IONS; MERR FAMILY; PROTEIN; GOLD; FRET; NANOPARTICLES; SELECTIVITY; MECHANISMS; SENSORS; CELLS;
D O I：
10.1016/j.snb.2017.12.056
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In this study, we employed CupR protein in the construct of a fluorescence resonance energy transfer (FRET)-based protein biosensor for the detection of silver ions. We fused the CFP and the YPet through a truncated CupR protein (containing a dimerization helix and a metal binding domain) to create a FRET based biosensor for the detection of silver ions. Various aqueous media (including tap water and pond water) were examined to determine the matrix effects and agarose gel was used to increase the feasibility of this portable biosensors. The resulting biosensor has demonstrated high sensitivity and selectivity to silver ions. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：784 / 788
页数：5
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