A graphene quantum dot-based method for the highly sensitive and selective fluorescence turn on detection of biothiols

被引：106

作者：

Wu, Zhenzhu ^{[1
]}

Li, Wenying ^{[1
,2
]}

Chen, Jian ^{[1
]}

Yu, Cong ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

TALANTA | 2014年 / 119卷

基金：

中国国家自然科学基金;

关键词：

Graphene quantum dots; Biothiol compound; Fluorescence; Biosensor; LABEL-FREE; COLORIMETRIC PROBES; EMERGENT NANOLIGHTS; GOLD NANOPARTICLES; MASS-SPECTROMETRY; AQUEOUS-SOLUTIONS; CARBON NANODOTS; GLUTATHIONE; CYSTEINE; HOMOCYSTEINE;

D O I：

10.1016/j.talanta.2013.11.065

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A facile assay method for the highly sensitive and selective sensing of biothiols based on graphene quantum dots (GQDs) has been developed. GQDs emitted strong blue fluorescence in an aqueous buffer solution. It was observed that mercury(II) ions could efficiently bind and quench the fluorescence of the GQDs. When a biothiol compound (glutathione, cysteine, or homocysteine) was added to the assay mixture of GQDs and mercury(II), it bound to mercury(II) ions. Hg2+-GQD complex dissociated, and a fluorescence turn-on signal was detected. The emission intensity changes of the GQDs could be directly related to the amount of biothiol added to the assay solution. The assay is highly sensitive, the limits of detection CLOD) for GSH, Cys and Hcy were 5 nM, 2.5 nM and 5 nM, respectively. The assay is also highly selective, a number of amino acids and proteins were tested, and little interference was observed. In addition, GSH standard recovery in serum samples was also demonstrated. We envision that our assay method could facilitate the biothiol quantification related biological and biomedical research. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：538 / 543

页数：6

共 57 条

[41] Ag Nanoparticle-decorated graphene quantum dots for label-free, rapid and sensitive detection of Ag+ and biothiols [J].