Identification of 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue from the sheath of Leptothrix cholodnii

被引：10
作者：
Makita, Hiroko
Nakahara, Yuichi
Fukui, Hiroyuki
Miyanoiri, Yohei
Katahira, Masato
Seki, Hiroko
Takeda, Minoru
Koizumi, Jun-ichi
机构：
[1] Yokohama Natl Univ, Fac Engn, Div Mat Sci & Chem Engn, Yokohama, Kanagawa 2408501, Japan
[2] Yokohama City Univ, Int Grad Sch Arts & Sci, Dept Supramol Biol, Yokohama, Kanagawa 2300045, Japan
[3] RIKEN, Genome Sci Ctr, Yokohama, Kanagawa 2300045, Japan
[4] Chiba Univ, Ctr Chem Anal, Chiba 2638522, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2006年 / 70卷 / 05期
基金：
日本科学技术振兴机构;
关键词：
Leptothrix cholodnii; 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid; sheath;
D O I：
10.1271/bbb.70.1265
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The sheath of Leptothrix cholodnii is a glycoconjugate composed of a polysaccharide and a peptide rich in cysteine. In this study, structural determination of the hydrazinolyzate of the sheath was carried out. Since the hydrazinolyzate is a polysaccharide incorporated with cysteine, it was S-derivatized with a thiol-specific fluorogenic reagent, 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F). Fluorescent fragments were purified by HPLC, and their structures were analyzed by mass spectrometry and NMR spectroscopy. The sheath was found to contain 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue.
引用
收藏
页码：1265 / 1268
页数：4
相关论文
共 12 条
[1]   CHARACTERIZATION OF EXTRACELLULAR MN-2+-OXIDIZING ACTIVITY AND ISOLATION OF AN MN-2+-OXIDIZING PROTEIN FROM LEPTOTHRIX-DISCOPHORA SS-1 [J].
ADAMS, LF ;
GHIORSE, WC .
JOURNAL OF BACTERIOLOGY, 1987, 169 (03) :1279-1285
[2]   ULTRASTRUCTURE AND CHEMICAL-COMPOSITION OF THE SHEATH OF LEPTOTHRIX-DISCOPHORA SP-6 [J].
EMERSON, D ;
GHIORSE, WC .
JOURNAL OF BACTERIOLOGY, 1993, 175 (24) :7808-7818
[3]   ISOLATION, CULTURAL MAINTENANCE, AND TAXONOMY OF A SHEATH-FORMING STRAIN OF LEPTOTHRIX-DISCOPHORA AND CHARACTERIZATION OF MANGANESE-OXIDIZING ACTIVITY ASSOCIATED WITH THE SHEATH [J].
EMERSON, D ;
GHIORSE, WC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (12) :4001-4010
[4]  
Krijt J, 2001, CLIN CHEM, V47, P1821
[5]   A COMPUTER-ASSISTED STRUCTURAL-ANALYSIS OF REGULAR POLYSACCHARIDES ON THE BASIS OF C-13-NMR DATA [J].
LIPKIND, GM ;
SHASHKOV, AS ;
KNIREL, YA ;
VINOGRADOV, EV ;
KOCHETKOV, NK .
CARBOHYDRATE RESEARCH, 1988, 175 (01) :59-75
[6]   Mycothiol biochemistry [J].
Newton, GL ;
Fahey, RC .
ARCHIVES OF MICROBIOLOGY, 2002, 178 (06) :388-394
[7]   STRUCTURE OF A NOVEL DISULFIDE OF 2-(N-ACETYLCYSTEINYL)AMIDO-2-DEOXY-ALPHA-D-GLUCOPYRANOSYL-MYO-INOSITOL PRODUCED BY STREPTOMYCES SP [J].
SAKUDA, S ;
ZHOU, ZY ;
YAMADA, Y .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (07) :1347-1348
[8]   THIOLS OF INTRACELLULAR PATHOGENS - IDENTIFICATION OF OVOTHIOL-A IN LEISHMANIA-DONOVANI AND STRUCTURAL-ANALYSIS OF A NOVEL THIOL FROM MYCOBACTERIUM-BOVIS [J].
SPIES, HSC ;
STEENKAMP, DJ .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 224 (01) :203-213
[9]   Polyphasic characterization of the genus Leptothrix: New descriptions of Leptothrix mobilis sp nov and Leptothrix discophora sp nov nom rev and emended description of Leptothrix cholodnii emend [J].
Spring, S ;
Kampfer, P ;
Ludwig, W ;
Schleifer, KH .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1996, 19 (04) :634-643
[10]   Structural analysis of the sheath of a sheathed bacterium, Leptothrix cholodnii [J].
Takeda, M ;
Makita, H ;
Ohno, K ;
Nakahara, Y ;
Koizumi, J .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2005, 37 (1-2) :92-98
12