Donor Amine Salt-Based Continuous in situ-Product Crystallization in Amine Transaminase-Catalyzed Reactions

被引：19

作者：

Huelsewede, Dennis ^{[1
]}

Dohm, Jan-Niklas ^{[1
]}

von Langermann, Jan ^{[1
]}

机构：

[1] Univ Rostock, Inst Chem, Biocatalyt Synth Grp, Albert Einstein Str 3 A, D-18059 Rostock, Germany

来源：

ADVANCED SYNTHESIS & CATALYSIS | 2019年 / 361卷 / 11期

关键词：

biocatalysis; in situ-product removal; crystallization; amine; salts; ASYMMETRIC-SYNTHESIS; CHIRAL AMINE; REACTION EQUILIBRIUM; MEMBRANE; CASCADE;

D O I：

10.1002/adsc.201900217

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The unfavorable reaction equilibrium of transaminase-catalyzed reactions is a major challenge for the efficient biocatalytic synthesis of chiral amines. In this study the synthetic utilization of a salt-based, continuous in situ-product crystallization is described to overcome the thermodynamic limit in amine transaminase-reactions using only the commonly used amine donor isopropylamine. The simultaneous dissolution of isopropylammonium 3,3-diphenylpropionate (donor salt) in combination with the crystallization of the product salt facilitates a thermodynamic shift of the continuous amine transaminase-catalyzed reaction. The main process necessity is a lower product salt solubility in comparison to the applied donor salt. This concept facilitates a stoichiometric use of isopropylamine in combination with a significantly lowered concentration of the amines in solution.

引用

页码：2727 / 2733

页数：7

共 33 条

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