Expert consensus regarding standardization of sample preparation for clotting time assays

被引：8

作者：

Ieko, Masahiro ^{[1
]}

Komiyama, Yutaka ^{[2
]}

Yamazaki, Satoshi ^{[3
]}

Katagiri, Hisako ^{[4
]}

Shimazu, Chisato ^{[5
]}

Naito, Sumiyoshi ^{[6
]}

Matsuda, Masato ^{[7
]}

Yuki, Yoichi ^{[8
]}

Miyata, Kazuyoshi ^{[9
]}

Arai, Rika ^{[10
]}

Kumano, Osamu ^{[11
]}

Emmi, Mari ^{[12
]}

Yoshida, Tatsuya ^{[13
]}

Tanaka, Hideaki ^{[14
]}

Okuhara, Toshihiko ^{[15
]}

Yamashita, Tetsuji ^{[16
]}

Sekine, Yasuji ^{[17
]}

Homma, Masaru ^{[18
]}

Kawai, Yohko ^{[19
]}

Tohyama, Kaoru ^{[20
]}

Ieko, Masahiro ^{[1
]}

Komiyama, Yutaka ^{[2
]}

Yamazaki, Satoshi ^{[3
]}

Katagiri, Hisako ^{[4
]}

Shimazu, Chisato ^{[5
]}

Naito, Sumiyoshi ^{[6
]}

Matsuda, Masato ^{[7
]}

Yuki, Yoichi ^{[8
]}

Miyata, Kazuyoshi ^{[9
]}

Arai, Rika ^{[10
]}

Kumano, Osamu ^{[11
]}

Emmi, Mari ^{[12
]}

Yoshida, Tatsuya ^{[13
]}

Tanaka, Hideaki ^{[14
]}

Okuhara, Toshihiko ^{[15
]}

Yamashita, Tetsuji ^{[16
]}

Sekine, Yasuji ^{[17
]}

Homma, Masaru ^{[18
]}

Kawai, Yohko ^{[19
]}

Tohyama, Kaoru ^{[20
]}

机构：

[1] Hlth Sci Univ Hokkaido, Sch Dent, Dept Internal Med, 1757 Kanazawa, Ishikari, Hokkaido 0610293, Japan

[2] Hokuriku Univ, Fac Hlth & Med Sci, Kanazawa, Ishikawa, Japan

[3] St Marianna Univ, Sch Med Hosp, Dept Clin Lab, Kawasaki, Kanagawa, Japan

[4] Keio Univ Hosp, Clin Lab, Tokyo, Japan

[5] Teikyo Univ, Dept Clin Lab Sci, Fac Med Technol, Tokyo, Japan

[6] Hlth Sci Univ Hokkaido Hosp, Dept Clin Lab, Ishikari, Hokkaido, Japan

[7] Niigata Univ Med & Dent Hosp, Lab Med Div, Niigata, Japan

[8] Kyoto Prefectural Univ Med, Fac Clin Lab, Kyoto, Japan

[9] Nippon Becton Dickinson Co Ltd, Tokyo, Japan

[10] Terumo Co Ltd, Gen Hosp Prod Grp, Tokyo, Japan

[11] Sysmex Corp, Prot Technol Engn 1, Kobe, Hyogo, Japan

[12] Sekisui Med Co Ltd, Business Management Unit, Diagnost Div, Prod Strategy Dept, Tokyo, Japan

[13] LSI Medience Corp, IVD Business Segment, Res & Dev Dept, Tokyo, Japan

[14] Fujirebio Inc, Tokyo, Japan

[15] Fukuyama Clin Lab, Fukuyama, Hiroshima, Japan

[16] LSI Medience Corp, Clin Lab Business Segment, Business Management Div, Tokyo, Japan

[17] Kohtohbiken Med Labs Inc, Tsukuba, Japan

[18] Mitsukoshi Hlth & Welf Fdn, Tokyo, Japan

[19] Int Univ Hlth & Welf, Sanno Affiliated Hosp, Tokyo, Japan

[20] Kawasaki Med Sch, Dept Lab Med, Kurashiki, Okayama, Japan

来源：

INTERNATIONAL JOURNAL OF HEMATOLOGY | 2020年 / 112卷 / 05期

关键词：

Clotting time assay; Plasma sample; Centrifugation condition; Sample store condition; RESIDUAL PLATELET COUNT; ROUTINE COAGULATION; BLOOD;

D O I：

10.1007/s12185-020-02983-x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Accurate clotting time assay results are vital, as the test is employed to indicate the amount of oral anticoagulant to be prescribed, while it is also used for screening the hemorrhagic and thrombotic diseases. The procedure chosen for preparation of a patient blood sample including centrifugation can contribute to significant differences in the results obtained. Thus, for the purpose of proposing a standardized method to appropriately prepare blood samples prior to assay, the Japanese Society of Laboratory Hematology organized the Working Group for Standardization of Sample Preparation for Clotting Time Assays (WG). Following reviews of previously announced guidelines and original experimental results, consensus was obtained by the WG, with the main findings as follows. (1) The recommended anticoagulant in the blood collection tube is sodium citrate solution at 0.105-0.109 M (3.13-3.2%). (2) Whole blood samples should be stored at room temperature (18-25 degrees C) within 1 h of collection from the patient. (3) For plasma preparation, centrifugation at 1500 x gshould be performed for at least 15 min or at 2000 x gfor at least 10 min at room temperature. (4) After the plasma sample is prepared, it should be stored at room temperature and assayed within 4 h.

引用

页码：614 / 620

页数：7

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