Ethanol exerts anti-inflammatory effects in human adipose tissue in vitro

被引：14
作者：
Wandler, Anne [1 ]
Bruun, Jens M. [1 ]
Nielsen, Maria P. [1 ]
Richelsen, Bjorn [1 ]
机构：
[1] Aarhus Univ Hosp, Dept Endocrinol & Metab C, DK-8000 Aarhus C, Denmark
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2008年 / 296卷 / 1-2期
基金：
英国医学研究理事会;
关键词：
Ethanol; Adipokines; Inflammation; Adipose tissue (human); Macrophages;
D O I：
10.1016/j.mce.2008.09.006
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Moderate alcohol consumption is suggested to be associated with reduced inflammation and morbidity. Human adipose tissue (AT) and obesity is characterised by low-grade inflammation, so the present study wanted to investigate the effects of ethanol on inflammation in human AT in vitro. Subcutaneous human AT was incubated with ethanol [11-88 mM] under non-or LPS-stimulated [50 mg/mL] conditions. Protein and mRNA levels of adiponectin, IL-6, IL-8, TNF-alpha, MCP-1, and CD68 were assessed using ELISA and real-time RT-PCR, respectively. Non-stimulated. ethanol incubations up to 24h increased adiponectin release and mRNA expression (p<0.01) and decreased IL-6 release in both short-term [1.5h] (p<0.05) and long-term [24h] (p<0.01)incubations. Ethanol decreased LPS-stimulated IL-6, IL-8,TNF-alpha, and MCP-1 dose-dependently (all p < 0.01). Ethanol decreased CD68 mRNA (p, < 0.001),which correlated with the investigated adipokines (p < 0.05) but not adiponectin (p > 0.05). In conclusion. ethanol exerts anti-inflammatory effects in human AT, Suggesting that ethanol may attenuate whole-body inflammation. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：26 / 31
页数：6
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