Auto-aligning stimulated emission depletion microscope using adaptive optics

被引：50

作者：

Gould, Travis J. ^{[1
]}

Kromann, Emil B. ^{[2
]}

Burke, Daniel ^{[3
]}

Booth, Martin J. ^{[3
,4
]}

Bewersdorf, Joerg ^{[1
,2
]}

机构：

[1] Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06510 USA

[2] Yale Univ, Sch Med, Dept Biomed Engn, New Haven, CT 06510 USA

[3] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England

[4] Univ Oxford, Ctr Neural Circuits & Behav, Oxford OX1 3SR, England

来源：

OPTICS LETTERS | 2013年 / 38卷 / 11期

基金：

英国工程与自然科学研究理事会; 英国惠康基金;

关键词：

FLUORESCENCE MICROSCOPY; STED MICROSCOPY; RESOLUTION; NANOSCOPY;

D O I：

10.1364/OL.38.001860

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Stimulated emission depletion (STED) microscopy provides diffraction-unlimited resolution in fluorescence microscopy. Imaging at the nanoscale, however, requires precise alignment of the depletion and excitation laser foci of the STED microscope. We demonstrate here that adaptive optics can be implemented to automatically align STED and confocal images with a precision of 4.3 +/- 2.3 nm. (C) 2013 Optical Society of America

引用

页码：1860 / 1862

页数：3

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