Degradation of chlorpyrifos in contaminated soil by immobilized laccase

In the present study, the embedding-crosslinking method was used to immobilize fungal laccase, and to determine the suitable conditions for immobilization by measuring various activities of the immobilized laccase. In addition, the immobilized laccase was further employed to repair chlorpyrifos-contaminated soil, and then the degradation rates of chlorpyrifos were measured under different conditions. Based on the results, the appropriate conditions for the method of embedding-crosslinking were found to be as follows: concentration of sodium alginate - 3 %, concentration of glutaraldehyde - 1 %, crosslinking time - 6h, volume of crude laccase - 60 ml, and immobilization time 4 h. After 48 h, the degradation rate of chlorpyrifos in soil could reach at least 70 % by the use of immobilized laccase that was prepared by the method of embedding-crosslinking under different environmental conditions.