Transmural pressure inhibits prorenin processing in juxtaglomerular cell

被引:9
作者
Ichihara, A
Suzuki, H
Miyashita, Y
Naitoh, M
Hayashi, M
Saruta, T [1 ]
机构
[1] Keio Univ, Sch Med, Dept Internal Med, Tokyo 160, Japan
[2] Saitama Med Coll, Dept Internal Med, Moroyama, Saitama 35004, Japan
关键词
mechanoreceptors; renin-angiotensin system; calcium channels; endoplasmic reticulum; adenosine; 3; 5 '-cyclic monophosphate;
D O I
10.1152/ajpregu.1999.277.1.R220
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Pressure control of renin secretion involves a complex integration of shear stress, stretch, and transmural pressure (TP). This study was designed to delineate TP control of renin secretion with minimal influence of shear stress or stretch and to determine its mechanism. Rat juxtaglomerular (JG) cells were applied to a TP-loading apparatus for 12 h. In cells conditioned with atmospheric pressure or atmospheric pressure + 30 mmHg, renin secretion rate (RSR) averaged 29.6 +/- 3.7 and 14.5 +/- 3.3% (P < 0.05, n = 8 cultures), respectively, and active renin content (ARC) averaged 47.3 +/- 4.6 and 38.4 +/- 3.4 ng of ANG I.h(-1).million cells(-1) (P < 0.05, n = 10 cultures), respectively. Total renin content and renin mRNA levels were unaffected by TP. The TP-induced decrease in RSR was prevented by Ca2+-free medium and the Ca2+ channel blocker verapamil and was attenuated by thapsigargin and caffeine, which deplete intracellular Ca2+ stores. Thapsigargin and caffeine, but not Ca2+-free medium or verapamil, prevented TP-induced decreases in ARC. The adenylate cyclase activator forskolin did not modulate TP-induced decreases in RSR or ARC. These findings suggest that TP not only stimulates Ca2+ influx but also inhibits prorenin processing through an intracellular Ca2+ store-dependent mechanism and thus inhibits active renin secretion by JG cells.
引用
收藏
页码:R220 / R228
页数:9
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