Two new colorimetric methods for early detection of vancomycin and oxacillin resistance in Staphylococcus aureus

被引:17
作者
Coban, AY
Bozdogan, B
Cihan, CC
Cetinkaya, E
Bilgin, K
Darka, O
Akgunes, A
Durupinar, B
Appelbaum, PC
机构
[1] Penn State Univ, Milton S Hershey Med Ctr, Dept Pathol, Hershey, PA 17033 USA
[2] Adnan Menderes Univ Hastanesi, Mikrobiyoloji & Klin Mikrobiyoloji AD, TR-09100 Aydin, Turkey
[3] Ondokuz Mayis Univ, Sch Med, Dept Microbiol & Clin Microbiol, TR-55139 Samsun, Turkey
关键词
D O I
10.1128/JCM.44.2.580-582.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We developed two colorimetric methods for the detection of vancomycin- and oxacillin-resistant Staphylococcus aureus in <= 6 h: (i) a nitrate reductase assay and (ii) a resazurin microplate method. MICs agreed with results obtained by CLI methods for oxacillin. However, detection of vancomycin resistance required a larger inoculum. These methods may be recommended for the detection of vancomycin- and oxacillin-resistant S. aureus.
引用
收藏
页码:580 / 582
页数:3
相关论文
共 12 条
[1]  
[Anonymous], 2002, MMWR MORB MORTAL WKL, V51, P902
[2]  
[Anonymous], 2002, MMWR MORB MORTAL WKL, V51, P565
[3]   Comparison of the Velogene Rapid MRSA Identification Assay, Denka MRSA-Screen Assay, and BBL Crystal MRSA ID system for rapid identification of methicillin-resistant Staphylococcus aureus [J].
Arbique, J ;
Forward, K ;
Haldane, D ;
Davidson, R .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2001, 40 (1-2) :5-10
[4]   Evaluation of Alamar colorimetric broth microdilution susceptibility testing method for staphylococci and enterococci [J].
Baker, CN ;
Tenover, FC .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (11) :2654-2659
[5]   Derivatives of a vancomycin-resistant Staphylococcus aureus strain isolated at Hershey Medical Center [J].
Bozdogan, B ;
Ednie, L ;
Credito, K ;
Kosowska, K ;
Appelbaum, PC .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (12) :4762-4765
[6]  
Centers for Disease Control and Prevention (CDC), 2004, MMWR Morb Mortal Wkly Rep, V53, P322
[7]  
FERRARO MJ, 2003, MANUAL CLIN MICROBIO, P208
[8]  
*FOOD DRUG ADM, 2003, CLASS 2 SPEC CONTR G
[9]   Low-level vancomycin resistance in Staphylococcus aureus -: A Australian perspective [J].
Howden, BP ;
Ward, PB ;
Johnson, PDR ;
Charles, PGP ;
Grayson, ML .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2005, 24 (02) :100-108
[10]  
National Committee for Clinical Laboratory Standards, 2000, METH DIL ANT SUSC TE