Fluid Flow and Cell Proliferation of Mesenchymal Adipose-Derived Stem Cells in Small-Scale, Stirred, Single-Use Bioreactors

被引：50

作者：

Kaiser, Stephan C. ^{[1
]}

Jossen, Valentin ^{[1
]}

Schirmaier, Carmen ^{[1
]}

Eibl, Dieter ^{[1
]}

Brill, Silke ^{[2
]}

van den Bos, Christian ^{[2
]}

Eibl, Regine ^{[1
]}

机构：

[1] Zurich Univ Appl Sci, Sch Life Sci & Facil Management, CH-8820 Wadenswil, Switzerland

[2] Lonza Cologne GmbH, D-50829 Cologne, Germany

来源：

CHEMIE INGENIEUR TECHNIK | 2013年 / 85卷 / 1-2期

关键词：

Adipose-derived stem cells; CFD model; Microcarrier; Paricle image velocimetry; Single-use spinner flask; CONSTRUCT DEVELOPMENT; SHEAR-STRESS; CULTURE; EXPANSION; DIFFERENTIATION; MICROCARRIERS; SUSPENSION; SYSTEM;

D O I：

10.1002/cite.201200180

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

The fluid flow and suspension characteristics inside small-scale, stirred, single-use bioreactors were investigated experimentally and by means of computational fluid dynamics. The required impeller speeds for homogenous suspension were determined for two microcarrier types. The shear stress level and turbulence distribution were predicted using a numerical model, which was verified by particle image velocimetry measurements. In subsequent cultivations of primary mesencymal adipose-derived stem cells, up to 31.4-fold expansion in cell number was achieved for serum concentrations as low as 5%.

引用

页码：95 / 102

页数：8

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