Use of the γ-H2AX assay to monitor DNA damage and repair in cancer research

被引：376

作者：

Ivashkevich, Alesia ^{[1
]}

Redon, Christophe E. ^{[2
]}

Nakamura, Asako J. ^{[3
]}

Martin, Roger F. ^{[1
]}

Martin, Olga A. ^{[1
,4
]}

机构：

[1] Peter MacCallum Canc Ctr, Lab Mol Radiat Biol, Melbourne, Vic, Australia

[2] NCI, Mol Pharmacol Lab, Ctr Canc Res, Bethesda, MD 20892 USA

[3] Osaka Med Coll, Dept Anat & Cell Biol, Osaka, Japan

[4] Peter MacCallum Canc Ctr, Dept Radiat Oncol, Melbourne, Vic, Australia

来源：

CANCER LETTERS | 2012年 / 327卷 / 1-2期

基金：

美国国家卫生研究院;

关键词：

gamma-H2AX; Double-strand breaks (DSBs); Translational research; Chemotherapy; Radiation therapy; Radiosensitivity; Biodosimetry; Imaging; DOUBLE-STRAND BREAKS; HISTONE H2AX PHOSPHORYLATION; MEASURE CHROMOSOMAL RADIOSENSITIVITY; PERIPHERAL-BLOOD LYMPHOCYTES; AUTOMATED BIODOSIMETRY TOOL; CORONARY CT ANGIOGRAPHY; IN-VIVO FORMATION; RADIATION SENSITIVITY; BIOLOGICAL DOSIMETRY; IONIZING-RADIATION;

D O I：

10.1016/j.canlet.2011.12.025

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Formation of gamma-H2AX in response to DNA double stranded breaks (DSBs) provides the basis for a sensitive assay of DNA damage in human biopsies. The review focuses on the application of gamma-H2AX-based methods to translational studies to monitor the clinical response to DNA targeted therapies such as some forms of chemotherapy, external beam radiotherapy, radionuclide therapy or combinations thereof. The escalating attention on radiation biodosimetry has also highlighted the potential of the assay including renewed efforts to assess the radiosensitivity of prospective radiotherapy patients. Finally the gamma-H2AX response has been suggested as a basis for an in vivo imaging modality. (c) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

页码：123 / 133

页数：11

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