Use of the γ-H2AX assay to monitor DNA damage and repair in cancer research

被引：376
作者：
Ivashkevich, Alesia [1 ]
Redon, Christophe E. [2 ]
Nakamura, Asako J. [3 ]
Martin, Roger F. [1 ]
Martin, Olga A. [1 ,4 ]
机构：
[1] Peter MacCallum Canc Ctr, Lab Mol Radiat Biol, Melbourne, Vic, Australia
[2] NCI, Mol Pharmacol Lab, Ctr Canc Res, Bethesda, MD 20892 USA
[3] Osaka Med Coll, Dept Anat & Cell Biol, Osaka, Japan
[4] Peter MacCallum Canc Ctr, Dept Radiat Oncol, Melbourne, Vic, Australia
来源：
CANCER LETTERS | 2012年 / 327卷 / 1-2期
基金：
美国国家卫生研究院;
关键词：
gamma-H2AX; Double-strand breaks (DSBs); Translational research; Chemotherapy; Radiation therapy; Radiosensitivity; Biodosimetry; Imaging; DOUBLE-STRAND BREAKS; HISTONE H2AX PHOSPHORYLATION; MEASURE CHROMOSOMAL RADIOSENSITIVITY; PERIPHERAL-BLOOD LYMPHOCYTES; AUTOMATED BIODOSIMETRY TOOL; CORONARY CT ANGIOGRAPHY; IN-VIVO FORMATION; RADIATION SENSITIVITY; BIOLOGICAL DOSIMETRY; IONIZING-RADIATION;
D O I：
10.1016/j.canlet.2011.12.025
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Formation of gamma-H2AX in response to DNA double stranded breaks (DSBs) provides the basis for a sensitive assay of DNA damage in human biopsies. The review focuses on the application of gamma-H2AX-based methods to translational studies to monitor the clinical response to DNA targeted therapies such as some forms of chemotherapy, external beam radiotherapy, radionuclide therapy or combinations thereof. The escalating attention on radiation biodosimetry has also highlighted the potential of the assay including renewed efforts to assess the radiosensitivity of prospective radiotherapy patients. Finally the gamma-H2AX response has been suggested as a basis for an in vivo imaging modality. (c) 2011 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：123 / 133
页数：11
相关论文
共 142 条
[1]   A novel splice variant of the DNA- PKcs gene is associated with clinical and cellular radiosensitivity in a patient with xeroderma pigmentosum [J].
Abbaszadeh, Fatemeh ;
Clingen, Peter H. ;
Arlett, Colin F. ;
Plowman, Piers N. ;
Bourton, Emma C. ;
Themis, Matthew ;
Makarov, Evgeny M. ;
Newbold, Robert F. ;
Green, Michael H. L. ;
Parris, Christopher N. .
JOURNAL OF MEDICAL GENETICS, 2010, 47 (03) :176-181
[2]   Strand breaks in plasmid DNA following positional changes of Auger-electron-emitting radionuclides [J].
Adelstein, SJ ;
Kassis, AI .
ACTA ONCOLOGICA, 1996, 35 (07) :797-801
[3]   Molecular imaging of cell death in vivo by a novel small molecule probe [J].
Aloya, Revital ;
Shirvan, Anat ;
Grimberg, Hagit ;
Reshef, Ayelet ;
Levin, Galit ;
Kidron, Dvora ;
Cohen, Avi ;
Ziv, Ilan .
APOPTOSIS, 2006, 11 (12) :2089-2101
[4]   The response of-H2AX in human lymphocytes and lymphocytes subsets measured in whole blood cultures [J].
Andrievski, Andrei ;
Wilkins, Ruth C. .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2009, 85 (04) :369-376
[5]   The use of IL-2 cultures to measure chromosomal radiosensitivity in breast cancer patients [J].
Baeyens, A ;
Vandenbulcke, K ;
Philippé, J ;
Thierens, H ;
De Ridder, L ;
Vral, A .
MUTAGENESIS, 2004, 19 (06) :493-498
[6]   The use of EBV-transformed cell lines of breast cancer patients to measure chromosomal radiosensitivity [J].
Baeyens, A ;
Thierens, H ;
Vandenbulcke, K ;
De Ridder, L ;
Vral, A .
MUTAGENESIS, 2004, 19 (04) :285-290
[7]   Epidermal growth factor receptor inhibition modulates the nuclear localization and cytotoxicity of the auger electron-emitting radiopharmaceutical 111In-DTPA-human epidermal growth factor [J].
Bailey, Kristy E. ;
Costantini, Danny L. ;
Cai, Zhongli ;
Scollard, Deborah A. ;
Chen, Zhuo ;
Reilly, Raymond M. ;
Vallis, Katherine A. .
JOURNAL OF NUCLEAR MEDICINE, 2007, 48 (09) :1562-1570
[8]   DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis [J].
Bartkova, J ;
Horejsi, Z ;
Koed, K ;
Krämer, A ;
Tort, F ;
Zieger, K ;
Guldberg, P ;
Sehested, M ;
Nesland, JM ;
Lukas, C ;
Orntoft, T ;
Lukas, J ;
Bartek, J .
NATURE, 2005, 434 (7035) :864-870
[9]   The Imaging of Apoptosis with the Radiolabelled Annexin A5: A New Tool in Translational Research [J].
Belhocine, Tarik Z. ;
Blankenberg, Francis G. .
CURRENT CLINICAL PHARMACOLOGY, 2006, 1 (02) :129-137
[10]   Preventing or reducing late side effects of radiation therapy: radiobiology meets molecular pathology [J].
Bentzen, Soren M. .
NATURE REVIEWS CANCER, 2006, 6 (09) :702-713
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