Comparative Transcriptome Profiling of Chilling Stress Responsiveness in Two Contrasting Rice Genotypes

被引：118

作者：

Zhang, Ting ^{[1
,2
]}

Zhao, Xiuqin ^{[2
]}

Wang, Wensheng ^{[2
]}

Pan, Yajiao ^{[2
]}

Huang, Liyu ^{[2
]}

Liu, Xiaoyue ^{[2
]}

Zong, Ying ^{[2
]}

Zhu, Linghua ^{[2
]}

Yang, Daichang ^{[1
]}

Fu, Binying ^{[2
]}

机构：

[1] Wuhan Univ, Coll Life Sci, Engn Res Ctr Plant Biotechnol & Germplasm Utiliza, Minist Educ,State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China

[2] Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China

来源：

PLOS ONE | 2012年 / 7卷 / 08期

关键词：

ORYZA-SATIVA L; COLD TOLERANCE; GENE-EXPRESSION; LOW-TEMPERATURE; SEEDLING STAGE; BOOTING STAGE; ABIOTIC STRESSES; MAJOR QTL; ARABIDOPSIS; RESPONSES;

D O I：

10.1371/journal.pone.0043274

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Rice is sensitive to chilling stress, especially at the seedling stage. To elucidate the molecular genetic mechanisms of chilling tolerance in rice, comprehensive gene expressions of two rice genotypes (chilling-tolerant LTH and chilling-sensitive IR29) with contrasting responses to chilling stress were comparatively analyzed. Results revealed a differential constitutive gene expression prior to stress and distinct global transcription reprogramming between the two rice genotypes under time-series chilling stress and subsequent recovery conditions. A set of genes with higher basal expression were identified in chilling-tolerant LTH compared with chilling-sensitive IR29, indicating their possible role in intrinsic tolerance to chilling stress. Under chilling stress, the major effect on gene expression was up-regulation in the chilling-tolerant genotype and strong repression in chilling-sensitive genotype. Early responses to chilling stress in both genotypes featured commonly up-regulated genes related to transcription regulation and signal transduction, while functional categories for late phase chilling regulated genes were diverse with a wide range of functional adaptations to continuous stress. Following the cessation of chilling treatments, there was quick and efficient reversion of gene expression in the chilling-tolerant genotype, while the chilling-sensitive genotype displayed considerably slower recovering capacity at the transcriptional level. In addition, the detection of differentially-regulated TF genes and enriched cis-elements demonstrated that multiple regulatory pathways, including CBF and MYBS3 regulons, were involved in chilling stress tolerance. A number of the chilling-regulated genes identified in this study were co-localized onto previously fine-mapped cold-tolerance-related QTLs, providing candidates for gene cloning and elucidation of molecular mechanisms responsible for chilling tolerance in rice.

引用

页数：12

共 80 条

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