SERUM QUANTIFICATION OF VITAMIN C BY HPLC-UV AND STABILITY STUDY

被引：8

作者：

Baierle, Marilia ^{[1
]}

de Bairros, Andre ^{[1
]}

Moreira, Ana Paula ^{[1
]}

Bulcao, Rachel ^{[1
]}

Roehrs, Miguel ^{[1
]}

de Freitas, Fernando ^{[1
]}

Durgante, Juliano ^{[1
]}

Brucker, Natalia ^{[1
]}

Charao, Mariele ^{[1
]}

Garcia, Solange Cristina ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, Fac Farm, Dept Anal, BR-90610000 Porto Alegre, RS, Brazil

来源：

QUIMICA NOVA | 2012年 / 35卷 / 02期

关键词：

HPLC; vitamin C; stability; PERFORMANCE LIQUID-CHROMATOGRAPHY; ASCORBIC-ACID; HUMAN-PLASMA; DEHYDROASCORBIC ACID; OXIDATIVE STRESS; RELIABLE BIOMARKERS; URIC-ACID; REPRODUCIBILITY; ANTIOXIDANTS; SAMPLES;

D O I：

10.1590/S0100-40422012000200030

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

SERUM QUANTIFICATION OF VITAMIN C BY HPLC-UV AND STABILITY STUDY. Vitamin C. an exogenous antioxidant, is essential to human health. In this study, a method was validated to serum vitamin C quantification by HPLC-UV. Its stability with and without the use of Iris [2-carboxy-ethyl] phosphine hydrochloride (TCEP), at -20 and -80 degrees C, in serum and supernatant were also evaluated. Analysis showed r(2) > 0.99, precision CV% < 15% and % bias < 15%, being linear, precise and accurate. The stability test revealed that using TCEP in serum storage at -20 and -80 degrees C or in supernatant at -80 degrees C the vitamin C levels remain stable for 30 and 12 days, respectively.

引用

页码：403 / 407

页数：5

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