Differentiation of lactic acid bacteria based on RFLP analysis of the tuf gene

被引：7

作者：

Park, Seok-Hwee ^{[1
,2
]}

Jung, Jong-Hyun ^{[1
,2
]}

Seo, Dong-Ho ^{[1
,2
]}

Lee, Hye-Lim ^{[1
,2
]}

Kim, Gye-Won ^{[3
,4
]}

Park, Sun-Young ^{[5
]}

Shin, Woo-Chang ^{[5
]}

Hong, Sungyoul ^{[6
]}

Park, Cheon-Seok ^{[1
,2
]}

机构：

[1] Kyung Hee Univ, Dept Food Sci & Biotechnol, Yongin 446701, Gyeonggi, South Korea

[2] Kyung Hee Univ, Inst Life Sci & Resources, Yongin 446701, Gyeonggi, South Korea

[3] Hankyong Natl Univ, Acad Ind Cooperat, Anseong 456749, Gyeonggi, South Korea

[4] Hankyong Natl Univ, Brewing Res Ctr, Anseong 456749, Gyeonggi, South Korea

[5] Kooksoondang Brewery Co Ltd, Res Lab, Seoul 462120, South Korea

[6] Sungkyunkwan Univ, Dept Genet Engn, Suwon 440746, Gyeonggi, South Korea

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2012年 / 21卷 / 03期

关键词：

Lactobacillus; tuf gene; restriction fragment length polymorphism (RFLP); lactic acid bacteria; FRAGMENT-LENGTH-POLYMORPHISM; COAGULASE-NEGATIVE STAPHYLOCOCCI; COMPARATIVE SEQUENCE-ANALYSIS; RIBOSOMAL-RNA GENES; LACTOBACILLUS-CASEI; SPECIES-LEVEL; IDENTIFICATION; PCR; PARACASEI; DIVERSITY;

D O I：

10.1007/s10068-012-0119-9

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The tuf gene, which encodes the elongation factor Tu, is universally distributed in Gram-positive bacteria. A small region of the tuf gene was evaluated for the differentiation of Lactobacillus strains using restriction fragment length polymorphism (RFLP) analysis. The 803 bp portion of tuf genes from 9 closely-related Lactobacillus reference strains were successfully amplified by polymerase chain reaction (PCR), and restriction patterns of each were evaluated with a set of restriction enzymes, which included AluI, HaeIII, and Sau3AI. The results implied that restriction patterns obtained from 2 of these restriction enzymes (AluI and HaeIII) could effectively differentiate closely-related Lactobacillus species.

引用

页码：911 / 915

页数：5

共 30 条

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