PURIFICATION AND IDENTIFICATION OF ANTIOXIDANT PEPTIDES FROM GELATIN HYDROLYSATES OF UNICORN LEATHERJACKET SKIN

被引：0

作者：

Karnjanapratum, S. ^{[1
]}

O'Callaghan, Y. C. ^{[2
]}

Benjakul, S. ^{[1
]}

O'Keeffe, M. B. ^{[3
]}

Fitzgerald, R. J. ^{[3
]}

O'Brien, N. M. ^{[2
]}

机构：

[1] Prince Songkla Univ, Fac Agroind, Dept Food Technol, Hat Yai 90112, Songkhla, Thailand

[2] Univ Coll Cork, Sch Food & Nutr Sci, Cork, Ireland

[3] Univ Limerick, Dept Life Sci, Limerick, Ireland

来源：

ITALIAN JOURNAL OF FOOD SCIENCE | 2017年 / 29卷 / 01期

关键词：

gelatin hydrolysate; unicorn leatherjacket; antioxidant activity; identification; UPLC; mass spectrometry; INHIBITORY PEPTIDES; BIOACTIVE PEPTIDES; PROTEIN; AUTOLYSIS; SEQUENCES; EXTRACT;

D O I：

暂无

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Antioxidant peptides from a gelatin hydrolysate of unicorn leatherjacket skin prepared using a partially purified glycyl endopeptidase were purified using Sephadex G-25 gel filtration, DEAE-cellulose anion-exchange and reverse phase high-performance liquid chromatography. The fractions with the highest ABTS radical scavenging activity were analyzed using UPLC-ESI-MS/MS to identify the peptide sequences therein. Four of the identified peptides, Glu-Pro-Gly-Pro-Val-Gly (555.27 Da), Leu-Pro-Gly-Pro-Ala-Gly (511.29 Da), Leu-Asp-Gly-Pro-Val-Gly (557.30 Da) and Glu-Gly-Pro-Leu-Gly (472.24 Da), were subsequently synthesized. Glu-Gly-Pro-Leu-Gly exhibited the highest antioxidant activity (4.95 mu mol TE/g solid). Therefore, peptides from unicorn leatherjacket skin gelatin hydrolysate could be further employed as functional food ingredient.

引用

页码：158 / 170

页数：13

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