PURIFICATION AND IDENTIFICATION OF ANTIOXIDANT PEPTIDES FROM GELATIN HYDROLYSATES OF UNICORN LEATHERJACKET SKIN

被引：0
|
作者：
Karnjanapratum, S. [1 ]
O'Callaghan, Y. C. [2 ]
Benjakul, S. [1 ]
O'Keeffe, M. B. [3 ]
Fitzgerald, R. J. [3 ]
O'Brien, N. M. [2 ]
机构：
[1] Prince Songkla Univ, Fac Agroind, Dept Food Technol, Hat Yai 90112, Songkhla, Thailand
[2] Univ Coll Cork, Sch Food & Nutr Sci, Cork, Ireland
[3] Univ Limerick, Dept Life Sci, Limerick, Ireland
来源：
ITALIAN JOURNAL OF FOOD SCIENCE | 2017年 / 29卷 / 01期
关键词：
gelatin hydrolysate; unicorn leatherjacket; antioxidant activity; identification; UPLC; mass spectrometry; INHIBITORY PEPTIDES; BIOACTIVE PEPTIDES; PROTEIN; AUTOLYSIS; SEQUENCES; EXTRACT;
D O I：
暂无
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Antioxidant peptides from a gelatin hydrolysate of unicorn leatherjacket skin prepared using a partially purified glycyl endopeptidase were purified using Sephadex G-25 gel filtration, DEAE-cellulose anion-exchange and reverse phase high-performance liquid chromatography. The fractions with the highest ABTS radical scavenging activity were analyzed using UPLC-ESI-MS/MS to identify the peptide sequences therein. Four of the identified peptides, Glu-Pro-Gly-Pro-Val-Gly (555.27 Da), Leu-Pro-Gly-Pro-Ala-Gly (511.29 Da), Leu-Asp-Gly-Pro-Val-Gly (557.30 Da) and Glu-Gly-Pro-Leu-Gly (472.24 Da), were subsequently synthesized. Glu-Gly-Pro-Leu-Gly exhibited the highest antioxidant activity (4.95 mu mol TE/g solid). Therefore, peptides from unicorn leatherjacket skin gelatin hydrolysate could be further employed as functional food ingredient.
引用
收藏
页码：158 / 170
页数：13
相关论文
共 50 条
  • [21] Optimisation of gelatin extraction from Unicorn leatherjacket (Aluterus monoceros) skin waste: response surface approach
    Hanjabam, Mandakini Devi
    Kannaiyan, Sathish Kumar
    Kamei, Gaihiamngam
    Jakhar, Jitender Kumar
    Chouksey, Mithlesh Kumar
    Gudipati, Venkateshwarlu
    JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, 2015, 52 (02): : 976 - 983
  • [22] Biomedical applications of gelatin from unicorn leatherjacket (Aluterus monoceros) skins
    Saah, S.
    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2014, 8 : 340 - 341
  • [23] Purification and identification of five novel antioxidant peptides from goat milk casein hydrolysates
    Li, Z.
    Jiang, A.
    Yue, T.
    Wang, J.
    Wang, Y.
    Su, J.
    JOURNAL OF DAIRY SCIENCE, 2013, 96 (07) : 4242 - 4251
  • [24] Purification and identification of novel antioxidant peptides derived from Bombyx mori pupae hydrolysates
    Chukiatsiri, Suttida
    Wongsrangsap, Nattakarn
    Kiatwuthinon, Pichamon
    Phonphoem, Wannarat
    BIOCHEMISTRY AND BIOPHYSICS REPORTS, 2024, 38
  • [25] Physico-mechanical and antimicrobial properties of gelatin film from the skin of unicorn leatherjacket incorporated with essential oils
    Ahmad, Mehraj
    Benjakul, Soottawat
    Prodpran, Thummanoon
    Agustini, Tri Winarni
    FOOD HYDROCOLLOIDS, 2012, 28 (01) : 189 - 199
  • [26] Indigenous proteases in the skin of unicorn leatherjacket (Alutherus monoceros) and their influence on characteristic and functional properties of gelatin
    Ahmad, Mehraj
    Benjakul, Soottawat
    Ovissipour, Mahmoudreza
    Prodpran, Thummanoon
    FOOD CHEMISTRY, 2011, 127 (02) : 508 - 515
  • [27] Purification and Identification of Novel Antioxidant Peptides from Hydrolysates of Peanuts (Arachis hypogaea) and Their Neuroprotective Activities
    Ma, Rui
    Bai, Jinrong
    Huang, Yuting
    Wang, Zhiqiu
    Xu, Yongju
    Huang, Yan
    Zhong, Kai
    Huang, Yina
    Gao, Hong
    Bu, Qian
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2023, 71 (15) : 6039 - 6049
  • [28] PURIFICATION AND IDENTIFICATION OF ANTIOXIDANT PEPTIDES FROM HYDROLYSATES OF LARGE YELLOW CROAKER (PSEUDOSCIAENA CROCEA) SCALES
    Chen, W.
    Hong, Y.
    Jia, Z.
    Huang, G.
    TRANSACTIONS OF THE ASABE, 2020, 63 (02) : 289 - 294
  • [29] Purification and identification of antioxidant peptides from chickpea (Cicer arietinum L.) albumin hydrolysates
    Kou, Xiaohong
    Gao, Jie
    Xue, Zhaohui
    Zhang, Zhijun
    Wang, Hua
    Wang, Xu
    LWT-FOOD SCIENCE AND TECHNOLOGY, 2013, 50 (02) : 591 - 598
  • [30] Preparation, purification, and identification of novel antioxidant peptides derived from Gracilariopsis lemaneiformis protein hydrolysates
    Hu, Xiao
    Liu, Jing
    Li, Jun
    Song, Yuqiong
    Chen, Shengjun
    Zhou, Shaobo
    Yang, Xianqing
    FRONTIERS IN NUTRITION, 2022, 9
12345