Over-expression of plant 14-3-3 proteins in tobacco: Enhancement of the plasmalemma K+ conductance of mesophyll cells

被引：42
|
作者：
Saalbach, G [1 ]
Schwerdel, M [1 ]
Natura, G [1 ]
Buschmann, P [1 ]
Christov, V [1 ]
Dahse, I [1 ]
机构：
[1] UNIV JENA,INST BIOCHEM & BIOPHYS,D-07743 JENA,GERMANY
来源：
FEBS LETTERS | 1997年 / 413卷 / 02期
关键词：
14-3-3; protein; Vicia faba cotyledon; tobacco transformation; covalent modification; ion channel activation; signal transduction;
D O I：
10.1016/S0014-5793(97)00865-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two cDNA clones encoding 14-3-3 homologous proteins were isolated from Vicia faba. Deduced amino acid sequences share different degrees of homology with other plant 14-3-3 proteins, Both clones, under the control of the CaMV 35S promoter, were transformed into tobacco plants, Immunoblotting showed three different forms of ca, 31, 34, and 37 kDa, indicating a covalent modification of the expressed 14-3-3 proteins, These forms were mainly present in the microsomal fraction, Patch-clamp studies of mesophyll protoplasts of the transformants revealed a strongly enhanced K+ conductance compared to the wild type, This indicates the involvement of 14-3-3 proteins in ion channel regulation, presumably by modulating kinase activities or binding the channel, (C) 1997 Federation of European Biochemical Societies.
引用
收藏
页码：294 / 298
页数：5
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