Effects of light and low oxygen tension on pigment biosynthesis in Halobacterium salinarum, revealed by a novel method to quantify both retinal and carotenoids

被引：28

作者：

El-Sayed, WSM

Takaichi, S

Saida, H

Kamekura, M

Abu-Shady, M

Seki, H

Kuwabara, T ^{[1
]}

机构：

[1] Univ Tsukuba, Inst Biol Sci, Tsukuba, Ibaraki 3058572, Japan

[2] Ain Shams Univ, Fac Sci, Dept Microbiol, Cairo, Egypt

[3] Nippon Med Coll, Biol Lab, Kawasaki, Kanagawa 2110063, Japan

[4] Toyama Univ Int Studies, Fac Reg Sci, Toyama 9301292, Japan

[5] Noda Inst Sci Res, Noda, Chiba 2780037, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2002年 / 43卷 / 04期

关键词：

bacterioruberin; hydroxylamine; low oxygen tension; retinal oxime; total beta-carotene synthesis;

D O I：

10.1093/pcp/pcf044

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A novel method for analyzing halobacterial pigments was developed, in which retinal was liberated from halobacterial rhodopsins as retinal oxime by hydroxylamine, ethyl beta-apo-8'-carotenoate was introduced as an internal standard, and the pigments including bacterioruberin and P-carotene were analyzed by HPLC at the same time. With this method, we revealed that light enhances the biosynthesis of bacterioruberin and the conversion of beta-carotene to retinal, but does not affect P-carotene biosynthesis in Halobacterium salinarum strain Oyon Moussa-16. Low oxygen tension given in the light brought a slight increase in retinal accumulation, although its biosynthesis from P-carotene is an oxygenation reaction. This paradox could be explained by the increase in beta-carotene biosynthesis.

引用

页码：379 / 383

页数：5

共 20 条

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DAVIES BH, 1988, PLANT PIGMENTS CRC H, V1, P1

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