Comparison of HPLC Methods for the Determination of Amino Sugars in Soil Hydrolysates

被引：11

作者：

Indorf, Caroline ^{[1
,3
]}

Bode, Samuel ^{[2
]}

Boeckx, Pascal ^{[2
]}

Dyckmans, Jens ^{[3
]}

Meyer, Axel ^{[4
]}

Fischer, Klaus ^{[4
]}

Joergensen, Rainer Georg ^{[1
]}

机构：

[1] Univ Kassel, Dept Soil Biol & Plant Nutr, D-37213 Witzenhausen, Germany

[2] Univ Ghent, Lab Appl Analyt & Phys Chem ISOFYS, B-9000 Ghent, Belgium

[3] Univ Gottingen, Ctr Stable Isotope Res & Anal, D-37073 Gottingen, Germany

[4] Univ Trier, Dept Analyt & Ecol Chem, Trier, Germany

来源：

ANALYTICAL LETTERS | 2013年 / 46卷 / 14期

关键词：

Amino sugars; Derivatization; HPAEC; HPLC; Microbial residues; OPA; PAD; Soil analysis; ANION-EXCHANGE CHROMATOGRAPHY; PERFORMANCE LIQUID-CHROMATOGRAPHY; PULSED AMPEROMETRIC DETECTION; MURAMIC ACID; DELTA-C-13; ANALYSIS; PUSHING AGENTS; GLUCOSAMINE; GALACTOSAMINE; FLUORESCENCE; MANNOSAMINE;

D O I：

10.1080/00032719.2013.796558

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A study on the suitability of chromatographic techniques such as high performance anion exchange chromatography (HPAEC) with fluorescence detection (FL) and pulsed amperometric detection (PAD) and reversed phase (RP) chromatography for the determination of galactosamine, glucosamine, mannosamine, and muramic acid in soil hydrolysates was carried out. The reversed phase fluorescence method was rapid, provided good validation parameters, and employed relatively inexpensive instrumentation. The HPAEC methods had slightly higher limits of quantification, 0.6-5.0 mu molL(-1) (HPAEC-FL) and 1.0-10.0 mu molL(-1) (HPAEC-PAD), compared to the reversed phase fluorescence method (0.5-5.0 mu molL(-1)). Various sample pretreatment methods and chromatographic methods were investigated and the advantages and disadvantages of the HPLC methods are discussed.

引用

页码：2145 / 2164

页数：20

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