Tenuifolin, a saponin derived from Radix Polygalae, exhibits sleep-enhancing effects in mice

被引:71
作者
Cao, Qing [1 ]
Jiang, Yong [2 ]
Cui, Su-Ying [1 ]
Tu, Peng-Fei [2 ]
Chen, Yue-Mei [2 ]
Ma, Xiao-Li [2 ]
Cui, Xiang-Yu [1 ]
Huang, Yuan-Li [1 ]
Ding, Hui [1 ]
Song, Jin-Zhi [1 ]
Yu, Bin [1 ]
Sheng, Zhao-Fu [1 ]
Wang, Zi-Jun [1 ]
Xu, Ya-Ping [1 ]
Yang, Guang [1 ]
Ye, Hui [1 ]
Hu, Xiao [1 ]
Zhang, Yong-He [1 ]
机构
[1] Peking Univ, Dept Pharmacol, Sch Basic Med Sci, 38 Xueyuan Rd, Beijing 100191, Peoples R China
[2] Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
基金
中国国家自然科学基金;
关键词
Polygala tenuifolia; Tenuifolin; Hypnotic; EEG; GABA; Noradrenaline; REM-SLEEP; IN-VITRO; RATS; POLYGALASAPONINS; ACTIVATION; WAKEFULNESS; COMPONENTS; BEHAVIORS; RECEPTORS; GABOXADOL;
D O I
10.1016/j.phymed.2016.10.015
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Background: Radix Polygalae, the dried root of Polygala tenuifolia, has been extensively used as a traditional Chinese medicine for promoting intelligence and tranquilization. Polygalasaponins extracted from the root of P. tenuifolia possess evident anxiolytic and sedative-hypnotic activities. Previous studies have reported that tenuifolin was a major constituent of polygalasaponins. Purpose: The currently study aims to investigate the hypnotic effect and possible mechanism of tenuifolin in freely moving mice. Design/Methods: The hypnotic effects of tenuifolin (20, 40 and 80 mg/kg, p. o.) were assessed by electroencephalographic (EEG) and electromyographic (EMG) analysis. Double-staining immunohistochemistry test was performed to evaluate the neuronal activity of sleep-wake regulating brain areas. High performance liquid chromatograph-electrochemical detection (HPLC-ECD) and ultrafast liquid chromatography-mass spectrometry (UFLC-MS) were used for the detection of neurotransmitters. Locomotor activity was measured by Open-field Test. Results: Tenuifolin at doses of 40 and 80 mg/kg (p. o.) significantly prolonged the total sleep time by increasing the amount of non-rapid eye movement (NREM) and rapid eye movement (REM) sleep, associated with the significant increase in the bouts of episodes respectively. After administration of tenuifolin, the cortical EEG power spectral densities during NREM and REM sleep were similar to that of natural sleep (vehicle) and thus compatible with physiological sleep. Double-immunohistochemistry staining test showed that tenuifolin increased the c-Fos positive ratios of GABAergic NREM sleep-promoting neurons in ventrolateral preoptic area (VLPO), cholinergic REM sleep-promoting neurons in laterodorsal tegmental area (LDT) and pontomesencephalic tegmental area (PPT) and decreased the c-Fos positive ratios in wake-promoting neurons (locus coeruleus (LC) and perifornical area (Pef)). Neurotransmitter detections revealed that tenuifolin significantly reduced the noradrenaline (NA) levels in LC, VLPO, PPT and LDT, elevated the GABA levels in VLPO, LC and Pef and increased the acetylcholine (Ach) levels in LDT and PPT. In addition, tenuifolin did not cause any change to locomotor activity. Conclusion: Taken together, these results provide the first experimental evidence of the significant sleepenhancing effect of tenuifolin in mice. This effect appears to be mediated, at least in part, by the activation of GABAergic systems and/or by the inhibition of noradrenergic systems. Moreover, this study adds new scientific evidence and highlights the therapeutic potential of the medicinal plant P. tenuifolia in the development of phytomedicines with hypnotic properties. (C) 2016 Elsevier GmbH. All rights reserved.
引用
收藏
页码:1797 / 1805
页数:9
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