Protective Effects of Hydrogen Sulfide Against Chronic Alcohol Intake-Induced Left Ventricular Remodeling in Rats

被引：11

作者：

Zhou, Xiang ^{[1
]}

Lu, Xiang ^{[2
]}

Xu, Weiting ^{[1
]}

Chen, Jianchang ^{[1
]}

机构：

[1] Soochow Univ, Affiliated Hosp 2, Dept Cardiol, Suzhou 215004, Peoples R China

[2] Nanjing Med Univ, Affiliated Hosp 2, Dept Geriatr, Nanjing, Peoples R China

来源：

CARDIOVASCULAR DRUGS AND THERAPY | 2013年 / 27卷 / 03期

关键词：

Hydrogen sulfide; Alcohol; Left ventricular remodeling; Oxidative stress; Apoptosis; ISCHEMIA-REPERFUSION INJURY; OXIDATIVE STRESS; MYOCARDIAL APOPTOSIS; HEART-FAILURE; MATRIX-METALLOPROTEINASE; CARDIAC FIBROBLASTS; CARDIOPROTECTION; CARDIOMYOPATHY; OXYGEN; HYPERTROPHY;

D O I：

10.1007/s10557-013-6441-5

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

To investigate the protective effects of hydrogen sulfide (H2S) against chronic alcohol intake-induced left ventricular remodeling and explore the potential mechanisms involved. Rats were randomly divided into 4 groups: alcohol group, NaHS group, alcohol + NaHS group, and control group. The echocardiographic and morphometric studies were performed to assess left ventricular remodeling. Oxidative stress was evaluated by detecting MDA, GSH-Px, Tot-SOD, CuZn-SOD and Mn-SOD in the supernatant. Cardiomyocyte apoptotic rate was determined by flow cytometry with Annexin V/PI staining. Western blotting was conducted to detect the expression of Bcl-2 family of apoptosis regulator proteins. The echocardiographic and morphometric data indicated that H2S has protective effects against chronic alcohol intake-induced left ventricular remodeling. Our findings showed a significant increase in MDA level and decreases in GSH-Px, Tot-SOD, CuZn-SOD and Mn-SOD activities in the alcohol group compared to the control group, while in the alcohol + NaHS group, a significant decrease in MDA level and increases in GSH-Px, Tot-SOD, CuZn-SOD and Mn-SOD activities were found compared to the alcohol group. The apoptotic rate in the alcohol group was significantly higher than in the control group, whereas apoptotic rate in the alcohol + NaHS group was significantly lower than in the alcohol group. In addition, Bcl-2 and Bcl-xL expression was upregulated and Bax expression was downregulated in the alcohol + NaHS group compared to the alcohol group. Our study demonstrates that H2S protects against chronic alcohol intake-induced left ventricular remodeling via attenuating oxidative stress and apoptosis.

引用

页码：221 / 227

页数：7

共 31 条

[1] Increased myocardial apoptosis in patients with unfavorable left ventricular remodeling and early symptomatic post-infarction heart failure [J].

Abbate, A ;

Biondi-Zoccai, GGL ;

Bussani, R ;

Dobrina, A ;

Camilot, D ;

Feroce, F ;

Rossiello, R ;

Baldi, F ;

Silvestri, F ;

Biasucci, LM ;

Baldi, A .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2003, 41 (05) :753-760

[2] Pathophysiologic role of myocardial apoptosis in post-infarction left ventricular remodeling [J].

Abbate, A ;

Biondi-Zoccai, GGL ;

Baldi, A .

JOURNAL OF CELLULAR PHYSIOLOGY, 2002, 193 (02) :145-153

[3] Role of hydrogen sulfide in the cardioprotection caused by ischemic preconditioning in the rat heart and cardiac myocytes [J].

Bian, JS ;

Yong, QC ;

Pan, TT ;

Feng, ZN ;

Ali, MY ;

Zhou, SF ;

Moore, PK .

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2006, 316 (02) :670-678

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] Hydrogen Sulfide Mediates Cardioprotection Through Nrf2 Signaling [J].

Calvert, John W. ;

Jha, Saurabh ;

Gundewar, Susheel ;

Elrod, John W. ;

Ramachandran, Arun ;

Pattillo, Christopher B. ;

Kevil, Christopher G. ;

Lefer, David J. .

CIRCULATION RESEARCH, 2009, 105 (04) :365-U105

[6] Role of apoptosis in ventricular remodeling. [J].

Chandrashekhar Y. .

Current Heart Failure Reports, 2005, 2 (1) :18-22

[7] Involvement of reactive oxygen species in angiotensin II-induced endothelin-1 gene expression in rat cardiac fibroblasts [J].

Cheng, TH ;

Cheng, PY ;

Shih, NL ;

Chen, IB ;

Wang, DL ;

Chen, JJ .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2003, 42 (10) :1845-1854

[8] Cardiac remodeling-concepts and clinical implications: A consensus paper from an international forum on cardiac remodeling [J].

Cohn, JN ;

Ferrari, R ;

Sharpe, N .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2000, 35 (03) :569-582

[9] Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial function [J].

Elrod, John W. ;

Calvert, John W. ;

Morrison, Joanna ;

Doeller, Jeannette E. ;

Kraus, David W. ;

Tao, Ling ;

Jiao, Xiangying ;

Scalia, Rosario ;

Kiss, Levente ;

Szabo, Csaba ;

Kimura, Hideo ;

Chow, Chi-Wing ;

Lefer, David J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (39) :15560-15565

[10] Increased Myostatin Activity and Decreased Myocyte Proliferation in Chronic Alcoholic Cardiomyopathy [J].

Fernandez-Sola, Joaquim ;

Lluis, Meritxell ;

Sacanella, Emilio ;

Estruch, Ramon ;

Antunez, Emilia ;

Urbano-Marquez, Alvaro .

ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 2011, 35 (07) :1220-1229

← 1 2 3 4 →