A Targeted LC-MS/MS Method for the Simultaneous Detection and Quantitation of Egg, Milk, and Peanut Allergens in Sugar Cookies

被引：39
作者：
Boo, Chelsea C. [1 ]
Parker, Christine H. [1 ]
Jackson, Lauren S. [2 ]
机构：
[1] US FDA, Ctr Food Safety & Appl Nutr, 5001 Campus Dr, College Pk, MD 20740 USA
[2] US FDA, Ctr Food Safety & Appl Nutr, 6502 South Archer Rd, Bedford Pk, IL 60501 USA
来源：
JOURNAL OF AOAC INTERNATIONAL | 2018年 / 101卷 / 01期
关键词：
MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; MULTIALLERGEN DETECTION; PROCESSED PEANUTS; FOOD; QUANTIFICATION; PROTEINS; VALIDATION; PEPTIDES; ASSAYS;
D O I：
10.5740/jaoacint.17-0400
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Food allergy is a growing public health concern, with many individuals reporting allergies to multiple food sources. Compliance with food labeling regulations and prevention of inadvertent cross-contact in manufacturing requires the use of reliable methods for the detection and quantitation of allergens in processed foods. In this work, a novel liquid chromatography-tandem mass spectrometry multiple-reaction monitoring method for multiallergen detection and quantitation of egg, milk, and peanut was developed and evaluated in an allergen-incurred baked sugar cookie matrix. A systematic evaluation of method parameters, including sample extraction, concentration, and digestion, were optimized for candidate allergen peptide markers. The optimized method enabled the reliable detection and quantitation of egg, milk, and peanut allergens in sugar cookies, with allergen concentrations as low as 5 ppm allergen incurred ingredient.
引用
收藏
页码：108 / 117
页数：10
相关论文
共 34 条
  • [1] Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques
    Capelo, J. L.
    Carreira, R.
    Diniz, M.
    Fernandes, L.
    Galesio, M.
    Lodeiro, C.
    Santos, H. M.
    Vale, G.
    [J]. ANALYTICA CHIMICA ACTA, 2009, 650 (02) : 151 - 159
  • [2] Use of specific peptide biomarkers for quantitative confirmation of hidden allergenic peanut proteins Ara h 2 and Ara Ill 3/4 for food control by liquid chromatography-tandem mass spectrometry
    Careri, M.
    Costa, A.
    Elviri, L.
    Lagos, J. -B.
    Mangia, A.
    Terenghi, M.
    Cereti, A.
    Garoffo, L. Perono
    [J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 389 (06) : 1901 - 1907
  • [3] Current Understanding of Egg Allergy
    Caubet, Jean-Christoph
    Wang, Julie
    [J]. PEDIATRIC CLINICS OF NORTH AMERICA, 2011, 58 (02) : 427 - +
  • [4] Proteomics-based approach to detect and identify major allergens in processed peanuts by capillary LC-Q-TOF (MS/MS)
    Chassaigne, Hubert
    Norgaard, Jorgen V.
    van Hengel, Arjon J.
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (11) : 4461 - 4473
  • [5] Food allergen detection by mass spectrometry: the role of systems biology
    Croote, Derek
    Quake, Stephen R.
    [J]. NPJ SYSTEMS BIOLOGY AND APPLICATIONS, 2016, 2
  • [6] Protein modification by thermal processing
    Davis, PJ
    Williams, SC
    [J]. ALLERGY, 1998, 53 : 102 - 105
  • [7] Fæste CK, 2007, J AOAC INT, V90, P427
  • [8] Effect of Heat Treatment on the Quantitative Detection of Egg Protein Residues by Commercial Enzyme-Linked Immunosorbent Assay Test Kits
    Fu, Tong-Jen
    Maks, Nicole
    Banaszewski, Katie
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (08) : 4831 - 4838
  • [9] Presence of Undeclared Food Allergens in Cumin: The Need for Multiplex Methods
    Garber, Eric A. E.
    Parker, Christine H.
    Handy, Sara M.
    Cho, Chung Y.
    Panda, Rakhi
    Samadpour, Mansour
    Reynaud, Danica H.
    Ziobro, George C.
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2016, 64 (05) : 1202 - 1211
  • [10] Gomaa A., 2012, J NUTR FOOD SCI, VS9, P001, DOI DOI 10.4172/2155-9600.S4179-4001
1234