Decrease of the Regulatory T-Cell Population by Adoptive T-Cell Transfer in a Mouse Colorectal Cancer Transplant Model

被引：1

作者：

Matsumoto, Tsuguhiro

Kokura, Satoshi ^{[1
,2
]}

Ishikawa, Takeshi ^{[2
]}

Funaki, Jun ^{[2
]}

Adachi, Satoko ^{[2
]}

Mori, Koji ^{[2
]}

Sakamoto, Naoyuki ^{[3
]}

Katada, Kazuhiro

Kamada, Kazuhiro

Yagi, Nobuaki

Handa, Osamu

Takagi, Tomohisa

Uchiyama, Kazuhiko

Naito, Yuji

Yosikawa, Toshikazu ^{[2
]}

机构：

[1] Kyoto Prefectural Univ Med, Dept Mol Gastroenterol & Hepatol, Kamigyo Ku, Kyoto 6028566, Japan

[2] Kyoto Prefectural Univ Med, Dept Canc Immunoregulat, Kyoto 6028566, Japan

[3] Iseikai Hyakumanben Clin, Kyoto, Japan

来源：

ONCOLOGY RESEARCH | 2011年 / 19卷 / 12期

关键词：

Adoptive T-cell transfer (ACT); Regulatory T cells (Tregs); Cancer; Interferon-gamma (INF-gamma); TUMOR-INFILTRATING LYMPHOCYTES; FOSTERS IMMUNE PRIVILEGE; TGF-BETA; PERIPHERAL-BLOOD; DENDRITIC CELLS; IFN-GAMMA; IN-VIVO; ESOPHAGEAL CANCERS; OVARIAN-CANCER; CUTTING EDGE;

D O I：

10.3727/096504012X13340632812596

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We examined the effects of adoptive T-cell transfer (ACT) on the population of regulatory T cells (Tregs) in a mouse colorectal cancer transplant model. In an in vivo study, Treg populations in Balb/c mice colon26 transplant model after ACT were analyzed in peripheral blood, local lymph node, and tumor. In an in vitro study CD4(+) cells were cultured in medium containing TGF-beta to induce Tregs. LAK cells were added or not in this Treg induction system. Treg induction after coculture with LAK was investigated. We also studied the role of IFN-gamma in the mechanism of Treg induction. Tregs in the draining lymph nodes and tumor were significantly suppressed by ACT. The induction of Tregs in vitro was inhibited by coculture with LAK cells. Furthermore, Tregs in the cultured cells were significantly inhibited by addition of exogenous IFN-gamma. Moreover, Tregs were increased by addition of IFN-gamma mAb. ACT may decrease Tregs in tumor-bearing hosts. One of the mechanisms is considered to be IFN-gamma inhibiting the induction of Tregs.

引用

页码：543 / 554

页数：12