Molecular Cloning of Hemocyanin cDNA from Fenneropenaeus chinensis and Antimicrobial Analysis of Two C-terminal Fragments

被引：39

作者：

Qiu, Chuwen ^{[1
,2
]}

Sun, Jie ^{[3
]}

Liu, Mei ^{[1
]}

Wang, Baojie ^{[1
]}

Jiang, Keyong ^{[1
]}

Sun, Shujuan ^{[1
]}

Meng, Xiaolin ^{[1
,2
]}

Luo, Zuoyong ^{[1
,2
]}

Wang, Lei ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, Qingdao 266071, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100039, Peoples R China

[3] Shandong Peanut Res Inst, Qingdao 266100, Peoples R China

来源：

MARINE BIOTECHNOLOGY | 2014年 / 16卷 / 01期

基金：

国家高技术研究发展计划(863计划);

关键词：

Fenneropenaeus chinensis; Hemocyanin; Antimicrobial peptides (AMPs); Pichia pastoris; Recombinant expression; ANTIBACTERIAL PEPTIDE; PICHIA-PASTORIS; PENAEUS-MONODON; LUNG LAVAGE; SHRIMP; PURIFICATION; PROTEINS; IMMUNITY; EXPRESSION; EVOLUTION;

D O I：

10.1007/s10126-013-9519-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Peptides derived from shrimp hemocyanin have antimicrobial properties. This is the first report of hemocyanin cDNA (FCHc) cloned from Fenneropenaeus chinensis and recombinant expression of two C-terminal fragments. Based on sequence analysis of Fenneropenaeus chinensis hemocyanin FCHc, we subcloned two FCHc fragments by designing special primers. Two antimicrobial peptides (AMPs) were derived from FCHc (FCHc-C1 and FCHc-C2). The recombinant sequence of FCHc-C1 consisted of 207 bp encoding 69 amino acids and the recombinant sequence of FCHc-C2 consisted of 120 bp encoding 40 amino acids. The results of Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting indicated that recombinant FCHc-C1 and FCHc-C2 peptides (rFCHc-C1 and rFCHc-C2) were expressed successfully. An inhibition assay showed that FCHc-C1 and FCHc-C2 were anionic AMPs with antifungal and antibacterial activities.

引用

页码：46 / 53

页数：8

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