Neurotrophic factor mediated neuronal differentiation of human cord blood mesenchymal stem cells and their applicability to assess the developmental neurotoxicity

被引：13

作者：

Jahan, Sadaf ^{[1
,2
,3
]}

Kumar, Dipak ^{[1
,2
]}

Kumar, Ashvini ^{[4
]}

Rajpurohit, Chetan Singh ^{[1
,2
]}

Singh, Shripriya ^{[1
,2
]}

Srivastava, Akriti ^{[1
]}

Pandey, Ankita ^{[1
]}

Pant, A. B. ^{[1
,2
]}

机构：

[1] CSIR, Indian Inst Toxicol Res, Syst Toxicol & Hlth Risk Assessment Grp, 31 VishvigyanBhawan,MG Marg, Lucknow 226001, UP, India

[2] Acad Sci & Innovat Res AcSIR, AcSIR Coordinator Off, New Delhi, India

[3] CDRI, CSIR, Jankipuram Extens, Sitapur Rd, Lucknow 226031, UP, India

[4] Med Sci & Associate Hosp, Career Inst, IIM Rd, Lucknow, UP, India

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 482卷 / 04期

关键词：

Human umbilical cord blood; Mesenchymal stem cells; Nerve growth factor; Neurodegeneration; Brain; Neuronal induction; NERVE GROWTH-FACTOR; ADULT NEUROGENESIS; NEURAL STEM; THERAPY;

D O I：

10.1016/j.bbrc.2016.11.140

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plasticity and developmental capacity of stem cells have now been established as a promising tool to restore the degenerative disorders. The linearity differentiation of human mesenchymal stem cells (hMSCs) into adipogenic, chondrogenic, osteogenic and even in neuronal subtypes has been demonstrated. The number of xenobiotics such as dexamethasone, insulin, isobutyl 1-methyle xanthine and retinoic acid has been reported for the potential to differentiate hMSCs into neuronal subtypes. But, the applicability of indigenous neurotrophic factor-nerve growth factor (NGF) has not been explored for the purpose. Thus, the present investigations were carried out to study the NGF induced neuronal differentiation of hMSCs. Following the isolation, purification and characterization of hMSCs were allowed to differentiate into neuronal subtypes under the influence of NGF (50 ng/mL). At various concentrations of NGF, the neuronal makers were analysed at both mRNA and protein levels. Cells, exposed with NGF were showing the significant and gradual increase in the neuronal markers in differentiating cells. The magnitude of expression of markers was maximum at day 4 of differentiation. NGF at 50 ng/mL concentration was found to induce neuronal differentiation of hMSCs into neuronal subtypes. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：961 / 967

页数：7

共 22 条

[1]

[Anonymous], 2012, TRICINE SDS PAGE, P81, DOI [10.1007/978-1-61779-821-4, DOI 10.1007/978-1-61779-821-4]

[2] New Emerging Potentials for Human Wharton's Jelly Mesenchymal Stem Cells: Immunological Features and Hepatocyte-Like Differentiative Capacity [J].