Anti-tumour Effects of Exosomes in Combination with Cyclophosphamide and Polyinosinic-Polycytidylic Acid

被引:39
作者
Guo, F. [2 ]
Chang, C. K. [1 ]
Fan, H. H. [3 ]
Nie, X. X. [3 ]
Ren, Y. N. [3 ]
Liu, Y. Y. [3 ]
Zhao, L. H. [3 ]
机构
[1] Shanghai Hosp 6, Dept Haematol, Shanghai 200233, Peoples R China
[2] Soochow Univ, Suzhou, Jiangsu Prov, Peoples R China
[3] Shanghai Blood Ctr, Shanghai, Peoples R China
关键词
CYCLOPHOSPHAMIDE; POLY I:C; ANTI-CANCER AGENTS; CANCER; TUMOUR IMMUNOLOGY; REGULATORY T CELLS; EXOSOMES; TOLL-LIKE RECEPTOR;
D O I
10.1177/147323000803600623
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
We examined the anti-tumour activity of exosomes derived from dendritic cells (DCs) in combination with cyclophosphamide (CTX) and polyinosinic-polycytidylic acid sodium salt (poly I:C). DCs were pulsed with L1210 lymphocytic leukaemia cell antigen and lipopolysaccharide. The exosomes that the DCs secreted were purified. In vitro, the anti-tumour activity of exosomes was assessed by measuring their ability to induce spleen cell proliferation and the extent to which they induced spleen cells to kill L1210 cells. Poly I:C was able to induce DC maturation. DC-derived exosomes stimulated spleen cell proliferation and enhanced the cytotoxic effects of spleen cells in vitro. DC-derived exosomes, in combination with CTX and poly 1:C, suppressed L1210 tumour growth in vivo and gave the greatest prolongation of survival time in tumour-bearing DBA2 mice. These findings suggest that this combination of a tumour vaccine, a conventional anti-cancer agent and a promoter of DC maturation might be a useful anti-cancer therapy.
引用
收藏
页码:1342 / 1353
页数:12
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