Anti-tumour Effects of Exosomes in Combination with Cyclophosphamide and Polyinosinic-Polycytidylic Acid

被引：39

作者：

Guo, F. ^{[2
]}

Chang, C. K. ^{[1
]}

Fan, H. H. ^{[3
]}

Nie, X. X. ^{[3
]}

Ren, Y. N. ^{[3
]}

Liu, Y. Y. ^{[3
]}

Zhao, L. H. ^{[3
]}

机构：

[1] Shanghai Hosp 6, Dept Haematol, Shanghai 200233, Peoples R China

[2] Soochow Univ, Suzhou, Jiangsu Prov, Peoples R China

[3] Shanghai Blood Ctr, Shanghai, Peoples R China

来源：

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH | 2008年 / 36卷 / 06期

关键词：

CYCLOPHOSPHAMIDE; POLY I:C; ANTI-CANCER AGENTS; CANCER; TUMOUR IMMUNOLOGY; REGULATORY T CELLS; EXOSOMES; TOLL-LIKE RECEPTOR;

D O I：

10.1177/147323000803600623

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We examined the anti-tumour activity of exosomes derived from dendritic cells (DCs) in combination with cyclophosphamide (CTX) and polyinosinic-polycytidylic acid sodium salt (poly I:C). DCs were pulsed with L1210 lymphocytic leukaemia cell antigen and lipopolysaccharide. The exosomes that the DCs secreted were purified. In vitro, the anti-tumour activity of exosomes was assessed by measuring their ability to induce spleen cell proliferation and the extent to which they induced spleen cells to kill L1210 cells. Poly I:C was able to induce DC maturation. DC-derived exosomes stimulated spleen cell proliferation and enhanced the cytotoxic effects of spleen cells in vitro. DC-derived exosomes, in combination with CTX and poly 1:C, suppressed L1210 tumour growth in vivo and gave the greatest prolongation of survival time in tumour-bearing DBA2 mice. These findings suggest that this combination of a tumour vaccine, a conventional anti-cancer agent and a promoter of DC maturation might be a useful anti-cancer therapy.

引用

页码：1342 / 1353

页数：12

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