Application of surface plasmon resonance spectroscopy for adsorption studies of different types of components on poly(dimethylsiloxane)

被引：11

作者：

Gaspar, Attila ^{[1
]}

Gomez, Frank A. ^{[1
]}

机构：

[1] Calif State Univ Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90032 USA

来源：

ANALYTICA CHIMICA ACTA | 2013年 / 777卷

基金：

美国国家科学基金会;

关键词：

Surface plasmon resonance; Poly(dimethylsiloxane); Adsorption; Microchip; SELF-ASSEMBLED MONOLAYERS; PROTEIN ADSORPTION; PDMS MEMBRANE; ABSORPTION; POLYDIMETHYLSILOXANE; DEVICES; FLOW;

D O I：

10.1016/j.aca.2013.03.035

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Surface plasmon resonance (SPR) spectroscopy is utilized to study in real-time and, by label-free means, the reversible and quasi-irreversible adsorption of small ionic or neutral molecules, pharmaceuticals, and proteins on poly(dimethylsiloxane) (PDMS) surfaces. The SPR sensor is covered with 0.2% (w/v) PDMS in octane. During the timescale of a typical lab-on-a-chip analysis or an electrophoretic separation, it was found that small neutral components containing a hydrophobic part do not adsorb or absorb onto PDMS, while larger, water-soluble polymer-like materials like proteins generally irreversibly adsorb to PDMS. The technique can be used to monitor the kinetics of adsorption and desorption of the molecules. For the non-specific adsorption of teicoplanin to PDMS, a Langmuir-like adsorption isotherm was obtained (K-d = 32 +/- 2 mu moI L-1). (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：72 / 77

页数：6

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