Influence of glycerol on the structure and stability of ferric horse heart myoglobin: A SAXS and circular dichroism study
被引:18
作者:
Barteri, M
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UNIV ROMA TOR VERGATA,DIPARTIMENTO MED SPERIMENTALE & SCI BIOCHIM,I-00133 ROME,ITALYUNIV ROMA TOR VERGATA,DIPARTIMENTO MED SPERIMENTALE & SCI BIOCHIM,I-00133 ROME,ITALY
Barteri, M
[1
]
Gaudiano, MC
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UNIV ROMA TOR VERGATA,DIPARTIMENTO MED SPERIMENTALE & SCI BIOCHIM,I-00133 ROME,ITALYUNIV ROMA TOR VERGATA,DIPARTIMENTO MED SPERIMENTALE & SCI BIOCHIM,I-00133 ROME,ITALY
Gaudiano, MC
[1
]
Santucci, R
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UNIV ROMA TOR VERGATA,DIPARTIMENTO MED SPERIMENTALE & SCI BIOCHIM,I-00133 ROME,ITALYUNIV ROMA TOR VERGATA,DIPARTIMENTO MED SPERIMENTALE & SCI BIOCHIM,I-00133 ROME,ITALY
Santucci, R
[1
]
机构:
[1] UNIV ROMA TOR VERGATA,DIPARTIMENTO MED SPERIMENTALE & SCI BIOCHIM,I-00133 ROME,ITALY
来源:
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
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1996年
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1295卷
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01期
The influence of glycerol on the structural properties of Fe(III)-horse heart myoglobin has been investigated by absorbance, CD and SR-SAXS spectroscopy. The results obtained indicate that both the tertiary and the secondary (alpha-helix) conformations of the protein are influenced by glycerol; in particular, an increase of approx. 8% in helical content was observed. Further, analysis of both the acid- and guanidine-induced denaturation transitions points to a glycerol-induced decreased stability of the tertiary structure; conversely, the alpha-helix conformation is found to be stabilized by the organic solvent. Finally, the SR-SAXS data show that gyration radius, cross-section and thickness of the protein increase in the presence of the organic solvent; however, the protein maintains a compact state.
机构:
China Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R ChinaChina Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R China
Gu, Yunlei
Cui, Naiyun
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China Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R ChinaChina Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R China
Cui, Naiyun
Yu, Qingjun
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China Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R ChinaChina Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R China
Yu, Qingjun
Li, Chunyi
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China Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R ChinaChina Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R China
Li, Chunyi
Cui, Qiukai
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机构:
PetroChina Corp, Dagang Petrochem Co, Tianjin 300280, Peoples R ChinaChina Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R China
机构:
China Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R ChinaChina Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R China
Gu, Yunlei
Cui, Naiyun
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h-index: 0
机构:
China Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R ChinaChina Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R China
Cui, Naiyun
Yu, Qingjun
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机构:
China Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R ChinaChina Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R China
Yu, Qingjun
Li, Chunyi
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机构:
China Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R ChinaChina Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R China
Li, Chunyi
Cui, Qiukai
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机构:
PetroChina Corp, Dagang Petrochem Co, Tianjin 300280, Peoples R ChinaChina Univ Petr, State Key Lab Heavy Oil Proc, Qingdao 266555, Peoples R China