Generating a Non-Integrating Human Induced Pluripotent Stem Cell Bank from Urine-Derived Cells

被引：149
|
作者：
Xue, Yanting [1 ,2 ,3 ]
Cai, Xiujuan [1 ,3 ]
Wang, Linli [1 ,3 ]
Liao, Baojian [1 ,3 ]
Zhang, Hui [1 ,3 ]
Shan, Yongli [1 ,3 ]
Chen, Qianyu [1 ,3 ]
Zhou, Tiancheng [1 ,3 ]
Li, Xirui [1 ,3 ]
Hou, Jundi [1 ,2 ,3 ]
Chen, Shubin [1 ,3 ]
Luo, Rongping [1 ,3 ]
Qin, Dajiang [1 ,3 ]
Pei, Duanqing [1 ,2 ,3 ]
Pan, Guangjin [1 ,2 ,3 ]
机构：
[1] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, South China Inst Stem Cell Biol & Regenerat Med, CAS Key Lab Regenerat Biol, Guangzhou 510530, Guangdong, Peoples R China
[2] Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China
[3] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, South China Inst Stem Cell Biol & Regenerat Med, Guangdong Prov Key Lab Stem Cell & Regenerat Med, Guangzhou 510530, Guangdong, Peoples R China
来源：
PLOS ONE | 2013年 / 8卷 / 08期
基金：
中国国家自然科学基金;
关键词：
HUMAN FIBROBLASTS; DERIVATION; INDUCTION; PLASMID; MIR-302;
D O I：
10.1371/journal.pone.0070573
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Induced pluripotent stem cell (iPS cell) holds great potential for applications in regenerative medicine, drug discovery, and disease modeling. We describe here a practical method to generate human iPS cells from urine-derived cells (UCs) under feeder-free, virus-free, serum-free condition and without oncogene c-MYC. We showed that this approach could be applied in a large population with different genetic backgrounds. UCs are easily accessible and exhibit high reprogramming efficiency, offering advantages over other cell types used for the purpose of iPS generation. Using the approach described in this study, we have generated 93 iPS cell lines from 20 donors with diverse genetic backgrounds. The non-viral iPS cell bank with these cell lines provides a valuable resource for iPS cells research, facilitating future applications of human iPS cells.
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页数：7
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