Protein thermostability engineering

被引：103
作者：
Modarres, H. Pezeshgi [1 ,2 ,4 ]
Mofrad, M. R. [1 ,2 ,3 ]
Sanati-Nezhad, A. [4 ,5 ]
机构：
[1] Univ Calif Berkeley, Mol Cell Biomech Lab, Dept Bioengn, 208A Stanley Hall, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Mol Cell Biomech Lab, Dept Mech Engn, 208A Stanley Hall, Berkeley, CA 94720 USA
[3] Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA
[4] Univ Calgary, Dept Mech & Mfg Engn, BioMEMS & Bioinspired Microfluid Lab, 2500 Univ Dr NW, Calgary, AB T2N 1N4, Canada
[5] Univ Calgary, Ctr BioEngn Res & Educ, 2500 Univ Dr NW, Calgary, AB T2N 1N4, Canada
来源：
RSC ADVANCES | 2016年 / 6卷 / 116期
基金：
加拿大自然科学与工程研究理事会;
关键词：
MOLECULAR-DYNAMICS SIMULATIONS; SIDE-CHAIN INTERACTIONS; BACILLUS-CIRCULANS XYLANASE; CONSTRAINT NETWORK ANALYSIS; COLONY-STIMULATING FACTOR; AMINO-ACID; THERMAL-STABILITY; RATIONAL DESIGN; DIRECTED EVOLUTION; CRYSTAL-STRUCTURE;
D O I：
10.1039/c6ra16992a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The use of enzymes for industrial and biomedical applications is limited to their function at elevated temperatures. The principles of thermostability engineering need to be implemented for proteins with low thermal stability to broaden their applications. Therefore, understanding the thermal stability modulating factors of proteins is necessary for engineering their thermostability. In this review, first different thermostability enhancing strategies in both the sequence and structure levels, discovered by studying the natural proteins adapted to different conditions, are introduced. Next, the progress in the development of various computational methods to engineer thermostability of proteins by learning from nature and introducing several popular tools and algorithms for protein thermostability engineering is highlighted. Further discussion includes the challenges in the field of protein thermostability engineering such as the protein stability- activity trade- off. Finally, how thermostability engineering could be instrumental for the design of protein drugs for biomedical applications is demonstrated.
引用
收藏
页码：115252 / 115270
页数：19
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