Non-specific immune response and disease resistance induced by Siegesbeckia glabrescens against Vibrio parahaemolyticus in Epinephelus bruneus

被引：26

作者：

Harikrishnan, Ramasamy ^{[1
,2
]}

Kim, Dong-Hwi ^{[1
,2
]}

Hong, Seung-Hyun ^{[1
,2
]}

Mariappan, Pitchaimuthu ^{[3
]}

Balasundaram, Chellam ^{[4
]}

Heo, Moon-Soo ^{[1
,2
]}

机构：

[1] Jeju Natl Univ, Marine Appl Microbes & Aquat Organism Dis Control, Dept Aquat Biomed Sci, Sch Marine Biomed Sci,Coll Ocean Sci, Cheju 690756, South Korea

[2] Jeju Natl Univ, Marine & Environm Res Inst, Cheju 690756, South Korea

[3] Rajah Serfoji Govt Coll, Dept Zool, Thanjavur 613005, Tamil Nadu, India

[4] Bharathidasan Univ, Dept Anim Sci, Tiruchirappalli 620024, Tamil Nadu, India

来源：

FISH & SHELLFISH IMMUNOLOGY | 2012年 / 33卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Disease resistance; Epinephelus bruneus; Non-specific immunity; Siegesbeckia glabrescens; Vibrio parahaemolyticus; PARALICHTHYS-OLIVACEUS; TRIHERBAL EXTRACT; IN-VITRO; INNATE; GROUPER; LYSOZYME; SYSTEM; FISH; DIET; PATHOGENESIS;

D O I：

10.1016/j.fsi.2012.05.018

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The immunomodulatory effect of Siegesbeckia glabrescens extract-supplementation diets on innate immune response and disease resistance of kelp grouper, Epinephelus bruneus against Vibrio parahaemolyticus at weeks 1, 2, and 4 is reported. The serum lysozyme activity was significantly enhanced with any enriched diet from weeks 1-4 when compared to control diet (0%). The alternative complement haemolytic activities significantly were enhanced with all enriched diets on weeks 2 and 4 whereas the cellular reactive oxygen species (ROS) was significantly enhanced only with 1.0% and 2.0% diets. The reactive nitrogen intermediate (RNI) value was significantly enhanced with any enriched diet on weeks 2 and 4, but on first week it did not differ from control. The myeloperoxidase (MPO) production significantly increased with 1.0% and 2.0% diets from second week onwards; with other enriched diets the increase manifested on fourth week; but during first week it did not vary from that of the control with any enriched diet. The protection in terms of cumulative mortality was the least being 25% and 20% when fed with 1.0% and 2.0% diets. The present results indicate that feeding kelp grouper with S. glabrescens extract enriched diet at 1.0% and 2.0% levels significantly enhance the immunological parameters, increase the disease resistance and minimize the cumulative mortality in E. bruneus against V. parahaemolyticus. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：359 / 364

页数：6

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