ATP secretion from nerve trunks and Schwann cells mediated by glutamate

被引：35

作者：

Liu, GJ ^{[1
]}

Bennett, MR

机构：

[1] Univ Sydney, Dept Physiol, Neurobiol Lab, Sydney, NSW 2006, Australia

[2] Univ Sydney, Biomed Res Inst, Sydney, NSW 2006, Australia

来源：

NEUROREPORT | 2003年 / 14卷 / 16期

关键词：

AMPA receptor; anion transporter; bioluminescence; exocytosis;

D O I：

10.1097/00001756-200311140-00014

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

ATP release from rat sciatic nerves and from cultured Schwann cells isolated from the nerves was investigated using an online bioluminescence technique. ATP was released in relatively large amounts from rat sciatic nerve trunks during electrical stimulation. This release was blocked by the sodium channel inhibitor tetrodotoxin and the non-NMDA glutamate receptor blocker 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). Schwann cells isolated from the nerve trunks did not release ATP when electrically stimulated but did in response to glutamate in a concentration-dependent manner. Glutamate-stimulated ATP release was inhibited by specific non-competitive AMPA receptor antagonist GYKI 52466 and competitive non-NMDA receptor antagonist CNQX. Glutamate-stimulated ATP release was decreased by inhibition of anion transporter inhibitors by furosemide, cystic fibrosis transmembrane conductance regulator by glibenclamide and exocytosis by botulinum toxin A, indicating that anion transporters and exocytosis provide the main secretion mechanisms for ATP release from the Schwann cells.

引用

页码：2079 / 2083

页数：5

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