A highly sensitive and selective turn-on fluorogenic and colorimetric sensor based on pyrene-functionalized magnetic nanoparticles for Hg2+ detection and cell imaging

被引:28
作者
Chen, Ling [1 ]
Zheng, Baozhan [1 ]
Guo, Yong [1 ]
Du, Juan [1 ]
Xiao, Dan [1 ,2 ]
Bo, Lin [3 ]
机构
[1] Sichuan Univ, Coll Chem, Chengdu 610064, Peoples R China
[2] Sichuan Univ, Coll Chem Engn, Chengdu 610064, Peoples R China
[3] Sichuan Univ, West China Hosp, Regenerat Med Res Ctr, Chengdu 610041, Peoples R China
基金
中国国家自然科学基金;
关键词
Fluorescent sensor; Py-Si-Fe3O4@SiO2 NPs; Magnetic core/shell; Water samples and serum sample; Hg2+; Living cells; HG(II) IONS; FT-IR; MERCURY; FLUORESCENCE; CHEMOSENSOR; EXCIMER; PROBE; HYDROCARBONS; GLUTATHIONE; SPECTRA;
D O I
10.1016/j.talanta.2013.09.001
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this paper, a colorimetric and "turn-on" fluorescent sensor (Py-Si-Fe3O4@SiO2 NPs) for Hg2+ detection was designed with pyrene derivative covalently grafted onto the surface of magnetic core/shell Fe3O4@SiO2 nanoparticles using the silanol hydrolysis approach. The Py-Si-Fe3O4@SiO2 inorganic-organic hybrid material was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray power diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and fluorescence emission. The results of fluorescence spectra showed that the resultant multifunctional nanoparticles exhibited selective turn-on type fluorescence enhancement with Hg2+. In addition, the presence of magnetic Fe3O4 nanoparticles in the sensor Py-Si-Fe3O4@SiO2 NPs would also facilitate the magnetic separation of Hg2+-Py-Si-Fe3O4@SiO2 from the solution. The as-prepared chemosensor was also successfully applied to detect Hg2+ in environmental water samples and serum sample. Results from confocal laser scanning microscopy experiments demonstrated that this chemosensor was cell permeable and can be used as a fluorescent probe for monitoring Hg2+ in living cells. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:338 / 344
页数:7
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