Biohydrogenation of dietary n-3 PUFA and stability of ingested vitamin E in the rumen, and their effects on microbial activity in sheep

被引:76
作者
Chikunya, S
Demirel, G
Enser, M
Wood, JD
Wilkinson, RG
Sinclair, LA
机构
[1] Harper Adams Univ Coll, Sch Agr, ASRC, Newport TF10 8NB, Shrops, England
[2] Univ Bristol, Sch Vet Sci, Div Food Anim Sci, Bristol BS40 5DU, Avon, England
关键词
polyunsaturated fatty acids; vitamin E; microbial protein synthesis;
D O I
10.1079/BJN20031078
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
The present study investigated the susceptibility of dietary n-3 PUFA to ruminal biohydrogenation, the stability of ingested vitamin E in the rumen and the subsequent uptake of PUFA and vitamin E into plasma. Six cannulated sheep were assigned to six diets over five 33 d periods, in an incomplete 6 X 5 Latin square. The diets, based on dried grass, were formulated to supply 50 g fatty acids/kg DM using three lipid sources: Megalac(R) (calcium soap of palm fatty acid distillate; Volac Ltd, Royston, Herts., UK), linseed (formaldehyde-treated; Trouw Nutrition, Northwich, Ches., UK) and linseed-fish oil (formaldehyde-treated linseed + fish oil). The diets were supplemented with 100 or 500 mg alpha-tocopheryl acetate/kg DM. Fat source or level of vitamin E in the diet did not alter microbial activity in the rumen. Biohydrogenation of linoleic acid (18: 3n-6; 85-90 %), linolenic acid (18: 3n-3; 88-93 %), docosahexaenoic acid (22: 6n-3; 91 %) and EPA (20: 5n-3; 92 %) was extensive. Feeding formaldehyde-treated linseed elevated concentrations of 18: 3n-3 in plasma, whilst 22: 6n-3 and 20: 5n-3 were only increased by feeding the linseed-fish oil blend. Duodenal recovery of ingested vitamin E was high (range 0.79-0.92 mg/mg fed). High dietary vitamin E was associated with increased plasma alpha-tocopherol (2.57 v. 1.46 mug/ml for 500 and 100 mg alpha-tocopheryl acetate/kg DM respectively), although all concentrations were low. Plasma vitamin E levels, however, tended to decrease as the type and quantity of PUFA in the diet increased. The present study illustrates that nutritionally beneficial PUFA in both fish and linseed oils are highly susceptible to biohydrogenation in the rumen. Although alpha-tocopheryl acetate resisted degradation in the rumen, plasma vitamin E status remained deficient to borderline, suggesting either that uptake may have been impaired or metabolism post-absorption increased.
引用
收藏
页码:539 / 550
页数:12
相关论文
共 66 条
  • [1] Agricultural Research Council, 1980, NUTR REQ RUM LIV
  • [2] PREINTESTINAL DISAPPEARANCE OF VITAMIN-E IN RUMINANTS
    ALDERSON, NE
    MITCHELL, GE
    LITTLE, CO
    WARNER, RE
    TUCKER, RE
    [J]. JOURNAL OF NUTRITION, 1971, 101 (05) : 655 - &
  • [3] INCORPORATION OF N-3 FATTY-ACIDS OF FISH OIL INTO TISSUE AND SERUM-LIPIDS OF RUMINANTS
    ASHES, JR
    SIEBERT, BD
    GULATI, SK
    CUTHBERTSON, AZ
    SCOTT, TW
    [J]. LIPIDS, 1992, 27 (08) : 629 - 631
  • [4] Association of official analytical chemist, 1995, OFF METH AN
  • [5] ASTRUP HN, 1974, ACTA VET SCAND, V15, P451
  • [6] Reticulo-rumen biohydrogenation and the enrichment of ruminant edible products with linoleic acid conjugated isomers
    Bessa, RJB
    Santos-Silva, J
    Ribeiro, JMR
    Portugal, AV
    [J]. LIVESTOCK PRODUCTION SCIENCE, 2000, 63 (03): : 201 - 211
  • [7] BINNERTS WT, 1968, VET REC, V82, P470
  • [8] EFFECT OF LINSEED OIL SUPPLEMENTATION ON FEED DEGRADATION AND MICROBIAL SYNTHESIS IN THE RUMEN OF CILIATE-FREE AND REFAUNATED SHEEP
    BROUDISCOU, L
    POCHET, S
    PONCET, C
    [J]. ANIMAL FEED SCIENCE AND TECHNOLOGY, 1994, 49 (3-4) : 189 - 202
  • [9] RUMEN FERMENTATION INVITRO AS INFLUENCED BY LONG-CHAIN FATTY-ACIDS
    CHALUPA, W
    RICKABAUGH, B
    KRONFELD, DS
    SKLAN, D
    [J]. JOURNAL OF DAIRY SCIENCE, 1984, 67 (07) : 1439 - 1444
  • [10] Chilliard Y, 2001, LIVEST PROD SCI, V70, P31, DOI 10.1016/S0301-6226(01)00196-8