Chemosensitization and drug accumulation assays as complementary methods for the screening of multidrug resistance reversal agents

被引：16

作者：

Quesada, AR ^{[1
]}

Barbacid, MM ^{[1
]}

Mira, E ^{[1
]}

Aracil, M ^{[1
]}

Marquez, G ^{[1
]}

机构：

[1] PHARMACIA ANTIBIOT FARMA SA,RES CTR,E-28026 MADRID,SPAIN

来源：

CANCER LETTERS | 1996年 / 99卷 / 01期

关键词：

multidrug resistance; chemosensitization; resistance modifier agent; Verapamil; PSC; 833; Rhodamine; 123;

D O I：

10.1016/0304-3835(95)04044-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Two methods based on the reversion of adriamycin-resistance or the increase of Rhodamine 123 accumulation in a multidrug resistant (MDR) cell line have been simplified and adapted for the screening of MDR reversal agents. Both methods are carried out in microtiter plates, are highly sensitive and can be easily automated. In both assays verapamil and the cyclosporine derivative PSC 833 could be detected at concentrations lower than 1 and 0.05 mu M, respectively. Depending on the MDR cell line used, drugs exhibiting the collateral sensitivity phenomenon can be selected in the cytotoxicity assay, while interferences due to sample toxicity are easily avoided in the dye accumulation assay.

引用

页码：109 / 114

页数：6

共 23 条

[1]

BIEDLER JL, 1994, CANCER RES, V54, P666

[2]

Biedler June L., 1993, Proceedings of the American Association for Cancer Research Annual Meeting, V34, P578

[3] INFLUENCE OF DEXNIGULDIPINE-HCL ON RHODAMINE-123 ACCUMULATION IN A MULTIDRUG-RESISTANT LEUKEMIA-CELL LINE - COMPARISON WITH OTHER CHEMOSENSITIZERS [J].

BOER, R ;

HAAS, S ;

SCHODL, A .

EUROPEAN JOURNAL OF CANCER, 1994, 30A (08) :1117-1123

[4]

BOESCH D, 1991, CANCER RES, V51, P4226

[5] BIOCHEMISTRY OF MULTIDRUG-RESISTANCE MEDIATED BY THE MULTIDRUG TRANSPORTER [J].

GOTTESMAN, MM ;

PASTAN, I .

ANNUAL REVIEW OF BIOCHEMISTRY, 1993, 62 :385-427

[6]

GRANDI M, 1987, P AM ASSOC CANC RES, V28, P279

[7] ISOLATION AND CHARACTERIZATION OF A HUMAN-COLON ADENOCARCINOMA CELL-LINE RESISTANT TO DOXORUBICIN [J].

GRANDI, M ;

GERONI, C ;

GIULIANI, FC .

BRITISH JOURNAL OF CANCER, 1986, 54 (03) :515-518

[8] MODULATION OF ANTITUMOR DRUG-RESISTANCE - EXPERIMENTAL LABORATORY DATA AND RESULTS OF CLINICAL-EVALUATION [J].

HILL, BT .

CANCER TREATMENT REVIEWS, 1990, 17 (2-3) :197-202

[9]

HOMOLYA L, 1993, J BIOL CHEM, V268, P21493

[10] MONITORING OF RELATIVE MITOCHONDRIAL-MEMBRANE POTENTIAL IN LIVING CELLS BY FLUORESCENCE MICROSCOPY [J].

JOHNSON, LV ;

WALSH, ML ;

BOCKUS, BJ ;

CHEN, LB .

JOURNAL OF CELL BIOLOGY, 1981, 88 (03) :526-535

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