Synthesis of Ogura male sterile rapeseed (Brassica napus L) with cold tolerance by protoplast fusion and effects of atrazine resistance on seed yield

被引:0
作者
Heath, DW [1 ]
Earle, ED [1 ]
机构
[1] CORNELL UNIV,DEPT PLANT BREEDING & BIOMETRY,ITHACA,NY 14853
关键词
Brassica napus; Ogura cytoplasmic male sterility; protoplast fusion; cold tolerance;
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Twenty-one cold-tolerant, male sterile Brassica napus somatic hybrids were produced by protoplast fusion. The fusion partners were a cold-sensitive, Ogura cytoplasmic male sterile cauliflower inbred (B. oleracea var. botrytis inbred NY7642A) and a cold-tolerant, fertile canola-type B. rapa cv. Candle. Hybridity was confirmed by morphology, isozyme expression, flow cytometry, and DNA hybridization. Organellar analyses revealed a very strong bias for Brassica over Raphanus chloroplasts. Cold tolerance was confirmed by cold chamber studies and chloroplast DNA analyses. Good female fertility with 21.4+/-3.1 seeds/pod was observed in the field using natural pollination vectors. Total seed yield was significantly greater for the atrazine-sensitive somatic hybrids produced in this study than for atrazine-resistant isolines.
引用
收藏
页码:939 / 944
页数:6
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