Preparation and characterization of immobilization of lipase in amino-functionalized mesoporous silica

被引:0
作者
Wang, Chun-feng [1 ]
Zhou, Guo-wei [1 ]
Zhang, Lei [1 ]
机构
[1] Shandong Polytech Univ, Key Lab Fine Chem Univ Shandong, Jinan 250353, Shandong, Peoples R China
来源
NANOTECHNOLOGY AND ADVANCED MATERIALS | 2012年 / 486卷
关键词
Poly(ethylene glycol); mesoporous silica; (3-aminopropyl)triethoxysilane (APTES); lipase; immobilization; PORCINE-PANCREATIC LIPASE; MOLECULAR-SIEVES; ADSORPTION; SBA-15; REACTOR; MCM-41;
D O I
10.4028/www.scientific.net/AMR.486.187
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Porcine pancreatic lipase (PPL) was immobilized in ordered mesoporous silica materials, which were using PEG 2000 (poly(ethylene glycol)) and P123 as dual templates and synthesized via hydrothermal process. The materials were silylated by (3 aminopropyl)triethoxysilane (APTES) by postsynthesis-grafting method. Samples were characterized by XRD, TEM, and BET. The results showed that the 2-dimensional hexagonal mesoscopic structure kept unchanging after modification. As compared to lipase immobilized mesoporous silica, the loading amount and hydrolization activity of lipase immobilized on aminopropyl-grafted mesoporous silica increased, respectively.
引用
收藏
页码:187 / 192
页数:6
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