The Hippo effector Yorkie activates transcription by interacting with a histone methyltransferase complex through Ncoa6

被引:58
作者
Qing, Yun [1 ,2 ]
Yin, Feng [1 ]
Wang, Wei [1 ]
Zheng, Yonggang [1 ]
Guo, Pengfei [1 ]
Schozer, Frederick [3 ]
Deng, Hua [1 ]
Pan, Duojia [1 ]
机构
[1] Johns Hopkins Univ, Sch Med, Howard Hughes Med Inst, Dept Mol Biol & Genet, Baltimore, MD 21205 USA
[2] Johns Hopkins Univ, Sch Med, BCMB Grad Program, Baltimore, MD USA
[3] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA
来源
ELIFE | 2014年 / 3卷
基金
美国国家卫生研究院;
关键词
D O I
10.7554/eLife.02564
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The Hippo signaling pathway regulates tissue growth in Drosophila through the transcriptional coactivator Yorkie (Yki). How Yki activates target gene transcription is poorly understood. Here, we identify Nuclear receptor coactivator 6 (Ncoa6), a subunit of the Trithorax-related (Trr) histone H3 lysine 4 (H3K4) methyltransferase complex, as a Yki-binding protein. Like Yki, Ncoa6 and Trr are functionally required for Hippo-mediated growth control and target gene expression. Strikingly, artificial tethering of Ncoa6 to Sd is sufficient to promote tissue growth and Yki target expression even in the absence of Yki, underscoring the importance of Yki-mediated recruitment of Ncoa6 in transcriptional activation. Consistent with the established role for the Trr complex in histone methylation, we show that Yki, Ncoa6, and Trr are required for normal H3K4 methylation at Hippo target genes. These findings shed light on Yki-mediated transcriptional regulation and uncover a potential link between chromatin modification and tissue growth.
引用
收藏
页码:1 / 13
页数:13
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