Expression of Th1 and Th2 cytokine-associated transcription factors, T-bet and GATA-3, in the eutopic endometrium of women with endometriosis

被引：25

作者：

Chen, Peng ^{[1
]}

Zhang, Zhifang ^{[1
]}

Chen, Qi ^{[1
]}

Ren, Fang ^{[1
]}

Li, Tong ^{[1
]}

Zhang, Chiyuan ^{[1
]}

Wang, Danbo ^{[1
]}

机构：

[1] China Med Univ, Dept Obstet & Gynecol, Shengjing Hosp, Shenyang 110004, Peoples R China

来源：

ACTA HISTOCHEMICA | 2012年 / 114卷 / 08期

基金：

中国国家自然科学基金;

关键词：

T-bet; GATA-3; Human endometriosis; Endometrium; T-BET/GATA-3; RATIO; DISEASE; PATHOGENESIS; INFLAMMATION; CELLS;

D O I：

10.1016/j.acthis.2012.01.005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The objective of the present study was to evaluate the expression of T-box expressed in T cells (T-bet) and GATA binding protein 3 (GATA-3) in the eutopic endometrium from women with endometriosis. Endometrial tissues were collected from 20 women with laparoscopically confirmed endometriosis and 20 women without endometriosis. T-bet and GATA-3 expression was measured by quantitative real-time PCR (qPCR), immunohistochemistry and Western blot analysis. Eutopic endometrial tissues from patients with endometriosis expressed lower levels of T-bet mRNA and high levels of GATA-3 mRNA, leading to a significant lower T-bet/GATA-3 mRNA ratio (P < 0.05). Western blot analysis showed that the T-bet/GATA-3 protein ratio in endometriosis group was also statistically lower than that in the control group (P < 0.05). These results suggested that T-bet and GATA-3 may act as cytokine regulatory genes, and the Th2-specific transcription factor, GATA-3, probably plays an essential role in the immune response and the development of endometriosis. (c) 2012 Elsevier GmbH. All rights reserved.

引用

页码：779 / 784

页数：6

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