Simultaneous densitometric determination of artemisinin, artemisinic acid and arteannuin-B in Artemisia annua using reversed-phase thin layer chromatography

被引：34

作者：

Bhandari, P ^{[1
]}

Gupta, AP ^{[1
]}

Singh, B ^{[1
]}

Kaul, VK ^{[1
]}

机构：

[1] Inst Himalayan Bioresource Technol, Nat Plant Prod Div, Palampur 176061, Himachal Prades, India

来源：

JOURNAL OF SEPARATION SCIENCE | 2005年 / 28卷 / 17期

关键词：

arteannuin-B; Artemisia annua; artemisinic acid; artemisinin; densitometry; RP-TLC;

D O I：

10.1002/jssc.200500198

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid and simple RP-TLC method for simultaneous quantification of pharmacologically important sesquiterpene artemisinin (AM) together with its precursors arteannuin-B (AB) and artemisinic acid (AA) in the inflorescence, part of Artemisia annua plant has been developed. The RP-TLC of sesquiterpenes was performed on RP-18 F-254 S thin-layer chromatographic plates by developing in mobile phase, containing 0.2% TFA in water/ACN (35:65, v/v). The densitometric determination of AM, AB and AA was carried out after derivatization with anisaldehyde reagent at 426 nm in absorption-reflectance mode.

引用

页码：2288 / 2292

页数：5

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