Template Usage Is Responsible for the Preferential Acquisition of the K65R Reverse Transcriptase Mutation in Subtype C Variants of Human Immunodeficiency Virus Type 1

被引：70

作者：

Coutsinos, Dimitrios ^{[1
,2
,3
]}

Invernizzi, Cedric F. ^{[1
,3
]}

Xu, Hongtao ^{[1
]}

Moisi, Daniela ^{[1
]}

Oliveira, Maureen ^{[1
]}

Brenner, Bluma G. ^{[1
,2
,3
]}

Wainberg, Mark A. ^{[1
,2
,3
]}

机构：

[1] McGill Univ, AIDS Ctr, Montreal, PQ H3T 1E2, Canada

[2] McGill Univ, Lady Davis Inst Med Res, Sir Mortimer B Davis Jewish Gen Hosp, Dept Microbiol & Immunol, Montreal, PQ H3T 1E2, Canada

[3] McGill Univ, Lady Davis Inst Med Res, Sir Mortimer B Davis Jewish Gen Hosp, Dept Med, Montreal, PQ H3T 1E2, Canada

来源：

JOURNAL OF VIROLOGY | 2009年 / 83卷 / 04期

基金：

加拿大健康研究院;

关键词：

DRUG-RESISTANCE; IN-VITRO; STRAND TRANSFER; HIV-1; SUBTYPE; DNA; TENOFOVIR; POLYMERIZATION; EPIDEMIOLOGY; IMPACT; NAIVE;

D O I：

10.1128/JVI.01349-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We propose that a nucleotide template-based mechanism facilitates the acquisition of the K65R mutation in subtype C human immunodeficiency virus type 1 (HIV-1). Different patterns of DNA synthesis were observed using DNA templates from viruses of subtype B or C origin. When subtype C reverse transcriptase (RT) was employed to synthesize DNA from subtype C DNA templates, preferential pausing was seen at the nucleotide position responsible for the AAG-to-AGG K65R mutation. This did not occur when the subtype B RT and template were used. Template factors can therefore increase the probability of K65R development in subtype C HIV-1.

引用

页码：2029 / 2033

页数：5

共 24 条

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