Transforming peanut (Arachis hypogaea L.) : a simple in planta method

被引：0

作者：

Wang, Chuan Tang ^{[1
]}

Wang, Xiu Zhen ^{[1
]}

Tang, Yue Yi ^{[1
]}

Wu, Qi ^{[1
]}

Li, Gui Jie ^{[1
]}

Song, Guo Sheng ^{[1
]}

Yu, Hong Tao ^{[1
]}

Hui, Dong Qing ^{[1
]}

Guo, Bao Tai ^{[1
]}

机构：

[1] Shandong Peanut Res Inst, Qingdao 266100, Peoples R China

来源：

RESEARCH ON CROPS | 2013年 / 14卷 / 03期

关键词：

ELISA; node injection method; NPT II; PCR; peanut; RT-PCR; transformation; CULTURE;

D O I：

暂无

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

An easy-to-follow gene transformation system is of vital importance to peanut, not only for varietal improvement and functional analysis of genes, but also for the production of pharmaceuticals. In this communication, a simple node injection peanut transformation method was described. Agrobacterial tumefaciens suspension was injected into nodes on the cotyledonary branches of field-grown peanut plants at 30 days after sowing, and PCR-positive seeds were obtained. Integration and expression of the NPT II gene were further confirmed by sequencing of the PCR/RT-PCR products and NPT II ELISA.

引用

页码：850 / 854

页数：5

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