Attenuation of lipopolysaccharide-induced neuroinflammatory events in BV-2 microglial cells by Moringa oleifera leaf extract

被引:2
作者
Sivaprakasam, Gothai [1 ]
Ganesan, Palanivel [2 ,3 ]
Muniandy, Katyakyini [1 ]
Park, Shin-Young [2 ]
Cho, Duk-Yeon [2 ]
Kim, Joon-So [2 ]
Arulselvan, Palanisamy [1 ,4 ,5 ]
Choi, Dong-Kug [3 ,6 ]
机构
[1] Univ Putra Malaysia, Lab Vaccines & Immunotherapeut, Inst Biosci, Serdang 43400, Malaysia
[2] Konkuk Univ, Dept Biomed Chem, Coll Biomed & Hlth Sci, Chungju 27478, Chungbuk, South Korea
[3] Konkuk Univ, Nanotechnol Res Ctr, Chungju 27478, Chungbuk, South Korea
[4] Scigen Res & Innovat Pvt Ltd, Periyar Technol Business Incubator, Periyar Nagar 613403, Thanjavur, India
[5] Muthayammal Coll Arts & Sci, Muthayammal Ctr Adv Res, Namakkal 637408, Tamil Nadu, India
[6] Konkuk Univ, Dept Biotechnol, Coll Biomed & Hlth Sci, Chungju 27478, Chungbuk, South Korea
基金
新加坡国家研究基金会;
关键词
BV2 microglial cells; Moringa oleifera leaf extract; Neuro-inflammation; NF-κB signaling pathway; Pro-inflammatory cytokines;
D O I
10.4103/2221-1691.254604
中图分类号
R188.11 [热带医学];
学科分类号
摘要
Objective: To determine the anti-neuroinflammatory activity of Moringa oleifera leaf extract (MLE) under lipopolysaccharide stimulation of mouse murine microglia BV2 cells in vitro. Methods: The cytotoxicity effect of MLE was investigated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide assay. The inflammatory response of BV-2 cells were induced with lipopolysaccharide. The generation of nitric oxide levels was determined by using Griess assay and the level of pro-inflammatory cytokines (IL-1 beta, IL-6 and TNF-alpha) was evaluated by ELISA kit. The expression of iNOS, COX-2 as well as IB-a was carried out by immunoblot analysis. Results: MLE reduced the nitric oxide production in concentration-dependent manner, and maintained the viability of BV-2 microglial cells which indicated absence of toxicity. In addition, MLE repressed the activation of nuclear factor kappa B by arresting the deterioration of I kappa B-alpha, consequently resulted in suppression of cytokines expression such as COX-2 and iNOS. Conclusions: MLE inhibitory activities are associated with the inhibition of nuclear factor kappa B transcriptional activity in BV2 microglial cells. Thus MLE may offer a substantial treatment for neuroinflammatory diseases.
引用
收藏
页码:109 / 115
页数:7
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