Ultra-Specific Isolation of Circulating Tumor Cells Enables Rare-Cell RNA Profiling

被引：29

作者：

Jack, Rhonda M. ^{[1
,2
]}

Grafton, Meggie M. G. ^{[1
,2
]}

Rodrigues, Danika ^{[3
]}

Giraldez, Maria D. ^{[4
,5
]}

Griffith, Catherine ^{[1
]}

Cieslak, Robert ^{[1
]}

Zeinali, Mina ^{[1
,2
,6
]}

Sinha, Chandan Kumar ^{[7
]}

Azizi, Ebrahim ^{[8
]}

Wicha, Max ^{[8
]}

Tewari, Muneesh ^{[2
,3
,4
,5
,8
,9
]}

Simeone, Diane M. ^{[10
]}

Nagrath, Sunitha ^{[1
,2
]}

机构：

[1] Dept Chem Engn, 3074 HH Dow Bldg,2300 Hayward St, Ann Arbor, MI 48109 USA

[2] Biointerfaces Inst, North Campus Res Complex,2800 Plymouth Rd,Bldg 90, Ann Arbor, MI 48109 USA

[3] Dept Biomed Engn, 1107 Carl A Gerstacker Bldg,2200 Bonisteel,Blvd, Ann Arbor, MI 48109 USA

[4] Univ Michigan, Dept Internal Med, Div Hematol Oncol, 109 Zina Pitcher Pl,2061 BSRB SPC 2200, Ann Arbor, MI 48109 USA

[5] Univ Michigan, Dept Internal Med, Div Mol Med & Genet, 109 Zina Pitcher Pl,2061 BSRB SPC 2200, Ann Arbor, MI 48109 USA

[6] Heidelberg Univ, Fac Med, Theodor Kutzer Ufer 1-3, D-68167 Mannheim, Germany

[7] Univ Michigan 5309 CCC 5940, Dept Pathol, Michigan Ctr Translat Pathol, 400 E Med Ctr Dr, Ann Arbor, MI 48109 USA

[8] Univ Michigan Hlth Syst, Ctr Comprehens Canc, 1500 E Med Ctr Dr, Ann Arbor, MI 48109 USA

[9] Univ Michigan, Ctr Computat Med & Bioinformat, Room 2017,100 Washtenaw Ave, Ann Arbor, MI 48109 USA

[10] Univ Michigan Hlth Syst, Dept Surg, 1500 E Med Ctr Dr, Ann Arbor, MI 48109 USA

来源：

ADVANCED SCIENCE | 2016年 / 3卷 / 09期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

PANCREATIC-CANCER; GENE-EXPRESSION; SEPARATION;

D O I：

10.1002/advs.201600063

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The clinical potential of circulating tumor cells (CTCs) in managing cancer metastasis is significant. However, low CTC isolation purities from patient blood have hindered sensitive molecular assays of these rare cells. Described herein is the ultra-pure isolation of CTCs from patient blood samples and how this platform has enabled highly specific molecular (mRNA and miRNA) profiling of patient CTCs.

引用

页数：9

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