Identification of differentially expressed genes and signalling pathways in the ovary of higher and lower laying ducks

被引：14

作者：

Sun, Y. ^{[1
,2
,3
]}

Wu, Q. ^{[1
,2
,3
]}

Pan, J. ^{[1
]}

Li, T. ^{[1
]}

Liu, L. ^{[1
]}

Chen, D. ^{[1
]}

Zhang, X. ^{[1
]}

Chen, H. ^{[4
]}

Li, Y. ^{[1
,2
,3
]}

Lin, R. ^{[4
]}

机构：

[1] Longyan Univ, Coll Life Sci, Longyan, Fujian, Peoples R China

[2] Fujian Prov Key Lab Prevent & Control Anim Infect, Longyan, Fujian, Peoples R China

[3] Fujian Prov Univ, Longyan Univ, Key Lab Prevent Vet Med & Biotechnol, Longyan, Fujian, Peoples R China

[4] Agr Bur Xinluo Dist, Longyan Shan Ma Duck Original Breeding Farm, Longyan, Peoples R China

来源：

BRITISH POULTRY SCIENCE | 2020年 / 61卷 / 06期

关键词：

Duck; egg production; RNA-seq; signalling pathways; integrin beta family genes; TRANSCRIPTOMIC ANALYSIS; EGG-PRODUCTION; RNA; MECHANISMS; INTEGRINS;

D O I：

10.1080/00071668.2020.1792834

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

1. Ovarian transcriptomic profiling between birds showing high egg number (HEN) and birds of low egg number (LEN) in Longyan Shan-ma ducks at 71 weeks of age was carried out using Illumina Hiseq 2500 technology. 2. A total of 343 differentially expressed genes (DEGs, 269 upregulated and 74 downregulated) were identified between HEN and LEN ovaries. These DEGs were enriched in 30 Gene Ontology terms. Pathway functional analysis found that the DEGs were enriched in 10 metabolic pathways (P < 0.05), one of which was regulation of the actin cytoskeleton pathway (Q< 0.05). 3. Three integrin family genes,ITGB2, ITGB5andITGA8were differentially expressed in the RNA-seq and qPCR experiments. 4. The DEGs and signalling pathways identified in ovarian tissue in this study provide new insights into high egg production in Longyan Shan-ma duck.

引用

页码：609 / 614

页数：6

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