Generation of T cell receptor-retrogenic mice: improved retroviral-mediated stem cell gene transfer

被引:31
作者
Bettini, Matthew L. [1 ]
Bettini, Maria [1 ]
Nakayama, Maki [2 ]
Guy, Clifford S. [1 ]
Vignali, Dario A. A. [1 ]
机构
[1] St Jude Childrens Res Hosp, Dept Immunol, Memphis, TN 38105 USA
[2] Univ Colorado Denver, Barbara Davis Ctr Childhood Diabet, Aurora, CO USA
关键词
D O I
10.1038/nprot.2013.111
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
T he use of retrogenic mice offers a rapid and flexible approach to T cell receptor (TCRTCRTCR)-transgenic mice. By transducing bone marrow progenitor cells with a retrovirus that encodes a given TCRTCRTCR-a/b subunit, TCRTCRTCR-retrogenic mice can be generated in as few as 4-6 weeks, whereas conventional TCRTCRTCR transgenics can take 6 months or longer. In this updated protocol, we have increased the efficiency of the bone marrow transduction and bone marrow reconstitution compared with our previously published protocol. The main departure from the previous protocol is the implementation of spin transduction with the viral supernatant instead of coculture with the viral producer cell line. The changes in this protocol improve bone marrow viability, increase consistency of the bone marrow transduction and bone marrow engraftment, and they reduce the ratio of bone marrow donor mice to bone marrow recipients.
引用
收藏
页码:1837 / 1840
页数:4
相关论文
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