Influenza A virus infection in Brazilian swine herds following the introduction of pandemic 2009 H1N1

被引：30

作者：

Ciacci-Zanella, Janice Reis ^{[1
]}

Schaefer, Rejane ^{[1
]}

Gava, Danielle ^{[1
]}

Haach, Vanessa ^{[2
]}

Cantao, Mauricio Egidio ^{[1
]}

Coldebella, Arlei ^{[1
]}

机构：

[1] Embrapa Swine & Poultry, Hlth Anim Lab, BR-89700000 Concordia, SC, Brazil

[2] Univ Western Santa Catarina UNOESC, CNPq, PIBIC, BR-89600000 Joacaba, SC, Brazil

来源：

VETERINARY MICROBIOLOGY | 2015年 / 180卷 / 1-2期

关键词：

Influenza A virus; Serology; Oral fluid; Swine; Brazil; ORAL FLUID SAMPLES; TIME RT-PCR; UNITED-STATES; PIGS; SURVEILLANCE; SENSITIVITY; SUBTYPES; FARMS; H3N2;

D O I：

10.1016/j.vetmic.2015.08.021

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Influenza A virus (FLUAV) infections are endemic in pork producing countries worldwide but in Brazil it was not considered an important pathogen in pigs. Since the emergence of 2009 pandemic H1N1 (H1N1pdm) FLUAV, many outbreaks of respiratory disease were observed in pig herds. The aim of this study was to evaluate FLUAV infection in swine in 48 pig farms located in seven Brazilian states with previous reports of influenza-like signs by clinical, serological and virological cross-sectional studies. Serological results showed that pigs from all farms had anti-influenza antibodies by NP-ELISA. Antibodies to H3N2, H1N2 and H1N1pdm were detected by HI in pigs from 24 farms. Co-infection with two or more FLUAV subtypes was detected in pigs in seven of those 24 farms. Detection of FLUAV in nasal swabs and oral fluids by RT-qPCR indicated a global concordance >81% for the two biological samples. Moreover, our results show that H1N1pdm, H1N2 and H3N2 viruses are widespread in Brazilian pig herds. The monitoring of FLUAV emergence and evolution in pigs is urgent, as well the study of the pathogenesis of Brazilian isolates, aiming to control influenza in pigs. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：118 / 122

页数：5

共 24 条

[1]

[Anonymous], INFLUENZA RES TREAT

[2] Sense and sensitivity - designing surveys based on an imperfect test [J].

Cannon, RM .

PREVENTIVE VETERINARY MEDICINE, 2001, 49 (3-4) :141-163

[3] Prevalence of swine influenza virus subtypes on swine farms in the United States [J].

Choi, YK ;

Goyal, SM ;

Joo, HS .

ARCHIVES OF VIROLOGY, 2002, 147 (06) :1209-1220

[4] Active Surveillance for Influenza A Virus among Swine, Midwestern United States, 2009-2011 [J].

Corzo, Cesar A. ;

Culhane, Marie ;

Juleen, Kevin ;

Stigger-Rosser, Evelyn ;

Ducatez, Mariette F. ;

Webby, Richard J. ;

Lowe, James F. .

EMERGING INFECTIOUS DISEASES, 2013, 19 (06) :954-960

[5]

Cunha R.G., 1978, REV BRAS BIOL, P13

[6] Detection of Influenza A virus in porcine oral fluid samples [J].

Detmer, Susan E. ;

Patnayak, Devi P. ;

Jiang, Yin ;

Gramer, Marie R. ;

Goyal, Sagar M. .

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2011, 23 (02) :241-247

[7] Distribution of antibodies against influenza virus in pigs from farrow-to-finish farms in Minas Gerais state, Brazil [J].

Dias, Alessandra S. ;

Costa, Erica A. ;

Rajao, Daniela S. ;

Guedes, Roberto M. C. ;

Ciacci Zanella, Janice R. ;

Lobato, Zelia I. P. .

Influenza and Other Respiratory Viruses, 2015, 9 (03) :161-167

[8] Probability of detecting influenza A virus subtypes H1N1 and H3N2 in individual pig nasal swabs and pen-based oral fluid specimens over time [J].

Goodell, Christa K. ;

Prickett, John ;

Kittawornrat, Apisit ;

Zhou, Fanghong ;

Rauh, Rolf ;

Nelson, William ;

O'Connell, Cate ;

Burrell, Angela ;

Wang, Chong ;

Yoon, Kyoung-Jin ;

Zimmerman, Jeffrey J. .

VETERINARY MICROBIOLOGY, 2013, 166 (3-4) :450-460

[9]

Howden KJ, 2009, CAN VET J, V50, P1153

[10]

Kitikoon P, 2014, METHODS MOL BIOL, V1161, P295, DOI 10.1007/978-1-4939-0758-8_24

← 1 2 3 →